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Individual in-vitro sensitivities of human pancreatic carcinoma cell lines to photodynamic therapy

  • Roswell Park Cancer Institute

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

Photodynamic therapy (PDT) is a promising alternative in the treatment of pancreatic cancer in man, due to the low sensitivity of the normal pancreas to PDT as shown in preclinical studies. Investigations on four human pancreatic cancer lines (MIA PaCa-2, PaCa 1, PaCa 3, and CAPAN 2) in vitro demonstrated a considerable variety in PDT-sensitivity proportional to the degree of differentiation, which was related to photosensitizer-uptake (PhotofrinTM). The well differentiated pancreatic tumor line Capan 2 showed a close relationship between high cell density and increased PDT-resistance. The Photofrin uptake of Capan 2 at high cell densities could be increased by short trypsinization prior to photosensitizer exposure. The data supports the hypothesis that a complex intercellular organization reduces the cell surface available for photosensitizer uptake and may cause the relative PDT resistance of normal pancreatic tissues and highly differentiated tumors.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages43-51
Number of pages9
ISBN (Print)0819407917
StatePublished - 1992
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy - Los Angeles, CA, USA
Duration: Jan 20 1992Jan 21 1992

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1645
ISSN (Print)0277-786X

Conference

ConferenceOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy
CityLos Angeles, CA, USA
Period01/20/9201/21/92

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