Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Alan J. Lesse; Anthony A. Campagnari; William E. Bittner; Michael A. Apicella

doi:10.1016/0022-1759(90)90018-Q

Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Alan J. Lesse
, Anthony A. Campagnari
, William E. Bittner
, Michael A. Apicella

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

296 Scopus citations

Abstract

We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the 'mini-gel' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their M_r during electrophoresis and allowed estimation of M_r from a protein standard. Several species of LOS were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS.

Original language	English
Pages (from-to)	109-117
Number of pages	9
Journal	Journal of Immunological Methods
Volume	126
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(90)90018-Q
State	Published - Jan 24 1990

Keywords

Electrophoresis
Lipooligosaccharide
Lipopolysaccharide

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10.1016/0022-1759(90)90018-Q

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title = "Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis",

abstract = "We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the 'mini-gel' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their Mr during electrophoresis and allowed estimation of Mr from a protein standard. Several species of LOS were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS.",

keywords = "Electrophoresis, Lipooligosaccharide, Lipopolysaccharide",

author = "Lesse, \{Alan J.\} and Campagnari, \{Anthony A.\} and Bittner, \{William E.\} and Apicella, \{Michael A.\}",

year = "1990",

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doi = "10.1016/0022-1759(90)90018-Q",

language = "English",

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T1 - Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis

AU - Lesse, Alan J.

AU - Campagnari, Anthony A.

AU - Bittner, William E.

AU - Apicella, Michael A.

PY - 1990/1/24

Y1 - 1990/1/24

N2 - We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the 'mini-gel' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their Mr during electrophoresis and allowed estimation of Mr from a protein standard. Several species of LOS were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS.

AB - We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the 'mini-gel' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their Mr during electrophoresis and allowed estimation of Mr from a protein standard. Several species of LOS were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS.

KW - Electrophoresis

KW - Lipooligosaccharide

KW - Lipopolysaccharide

UR - https://www.scopus.com/pages/publications/0025059032

U2 - 10.1016/0022-1759(90)90018-Q

DO - 10.1016/0022-1759(90)90018-Q

M3 - Article

C2 - 2106001

AN - SCOPUS:0025059032

SN - 0022-1759

VL - 126

SP - 109

EP - 117

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1

ER -

Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Abstract

Keywords

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