In vitro detection of apoptosis in monocytes/macrophages infected with human coronavirus

Arlene R. Collins

doi:10.1128/CDLI.9.6.1392-1395.2002

In vitro detection of apoptosis in monocytes/macrophages infected with human coronavirus

Arlene R. Collins

Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

Human coronavirus (HCoV) strain 229E infection, but not HCoV strain OC43 infection, of monocytes/macrophages from healthy donors and patients with multiple sclerosis in remission resulted in increased apoptosis, as measured by DNA changes and annexin V staining. Apoptosis correlated with the differential release of infectious virus. HCoV strain 229E titers were 10^3.5 to 10⁶ 50% tissue culture-infective doses (TCID₅₀)/ml, and HCoV strain OC43 titers were only 10^1.2 to 10^2.7 TCID₅₀/ml.

Original language	English
Pages (from-to)	1392-1395
Number of pages	4
Journal	Clinical and Diagnostic Laboratory Immunology
Volume	9
Issue number	6
DOIs	https://doi.org/10.1128/CDLI.9.6.1392-1395.2002
State	Published - Nov 2002

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10.1128/CDLI.9.6.1392-1395.2002

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title = "In vitro detection of apoptosis in monocytes/macrophages infected with human coronavirus",

abstract = "Human coronavirus (HCoV) strain 229E infection, but not HCoV strain OC43 infection, of monocytes/macrophages from healthy donors and patients with multiple sclerosis in remission resulted in increased apoptosis, as measured by DNA changes and annexin V staining. Apoptosis correlated with the differential release of infectious virus. HCoV strain 229E titers were 103.5 to 106 50\% tissue culture-infective doses (TCID50)/ml, and HCoV strain OC43 titers were only 101.2 to 102.7 TCID50/ml.",

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AU - Collins, Arlene R.

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N2 - Human coronavirus (HCoV) strain 229E infection, but not HCoV strain OC43 infection, of monocytes/macrophages from healthy donors and patients with multiple sclerosis in remission resulted in increased apoptosis, as measured by DNA changes and annexin V staining. Apoptosis correlated with the differential release of infectious virus. HCoV strain 229E titers were 103.5 to 106 50% tissue culture-infective doses (TCID50)/ml, and HCoV strain OC43 titers were only 101.2 to 102.7 TCID50/ml.

AB - Human coronavirus (HCoV) strain 229E infection, but not HCoV strain OC43 infection, of monocytes/macrophages from healthy donors and patients with multiple sclerosis in remission resulted in increased apoptosis, as measured by DNA changes and annexin V staining. Apoptosis correlated with the differential release of infectious virus. HCoV strain 229E titers were 103.5 to 106 50% tissue culture-infective doses (TCID50)/ml, and HCoV strain OC43 titers were only 101.2 to 102.7 TCID50/ml.

UR - https://www.scopus.com/pages/publications/0036842775

U2 - 10.1128/CDLI.9.6.1392-1395.2002

DO - 10.1128/CDLI.9.6.1392-1395.2002

M3 - Article

C2 - 12414783

AN - SCOPUS:0036842775

SN - 1071-412X

VL - 9

SP - 1392

EP - 1395

JO - Clinical and Diagnostic Laboratory Immunology

JF - Clinical and Diagnostic Laboratory Immunology

IS - 6

ER -

In vitro detection of apoptosis in monocytes/macrophages infected with human coronavirus

Abstract

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