Impact of photobiomodulation in a patient-derived xenograft model of oral squamous cell carcinoma

Felipe Martins Silveira; Tuany Rafaeli Schmidt; Bruna Neumann; Clévia Rosset; Virgilio Gonzales Zanella; Gerson Schulz Maahs; Marco Antonio Trevizani Martins; Praveen Arany; Vivian Petersen Wagner; Márcio Ajudarte Lopes; Alan Roger Santos-Silva; Manoela Domingues Martins

doi:10.1111/odi.13967

Impact of photobiomodulation in a patient-derived xenograft model of oral squamous cell carcinoma

Felipe Martins Silveira
, Tuany Rafaeli Schmidt
, Bruna Neumann
, Clévia Rosset
, Virgilio Gonzales Zanella
, Gerson Schulz Maahs
, Marco Antonio Trevizani Martins
, Praveen Arany
, Vivian Petersen Wagner
, Márcio Ajudarte Lopes
, Alan Roger Santos-Silva
, Manoela Domingues Martins

Department of Oral Biology

Universidade Estadual de Campinas
Porto Alegre Clinics Hospital
Universidade Federal do Rio Grande do Sul
Hospital Santa Rita

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Background: Photobiomodulation therapy (PBMT) is an effective method for the prevention of oral mucositis. However, the effects of PBMT on oral squamous cell carcinoma (OSCC) have not yet been fully elucidated. This study aimed to evaluate the impact of PBMT in an OSCC-patient-derived xenograft (OSCC-PDX) model. Methods: BALB/c nude mice with OSCC-PDX models were divided into Control, without PBMT (n = 8); Immediate irradiation, PBMT since one week after tumor implantation (n = 6); and Late irradiation, PBMT after tumors reached 200 mm³ (n = 6). OSCC-PDX were daily irradiated (660 nm; 100 mW; 6 J/cm²; 0,2 J/point) for 12 weeks. The tumors were collected and submitted to volumetric, histological, immunohistochemistry, and cell cycle analysis. Results: No significant differences in the volumetric measurements (p = 0.89) and in the histopathological grade (p > 0.05) were detected between the groups. The immunohistochemical analysis of Ki-67 (p = 0.9661); H3K9ac (p = 0.3794); and BMI1 (p = 0.5182), and the evaluation of the cell cycle phases (p > 0.05) by flow cytometry also did not demonstrate significant differences between the irradiated and non-irradiated groups. Conclusion: In this study, PBMT did not impact the behavior of OSCC-PDX models. This is an important preclinical outcome regarding safety concerns of the use of PBMT in cancer patients.

Original language	English
Pages (from-to)	547-556
Number of pages	10
Journal	Oral Diseases
Volume	29
Issue number	2
DOIs	https://doi.org/10.1111/odi.13967
State	Published - Mar 2023

Keywords

low-level light therapy
oral mucositis
oral squamous cell carcinoma
photobiomodulation therapy

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10.1111/odi.13967

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@article{657b12f5d71f4d90ac7da13eef0cb957,

title = "Impact of photobiomodulation in a patient-derived xenograft model of oral squamous cell carcinoma",

abstract = "Background: Photobiomodulation therapy (PBMT) is an effective method for the prevention of oral mucositis. However, the effects of PBMT on oral squamous cell carcinoma (OSCC) have not yet been fully elucidated. This study aimed to evaluate the impact of PBMT in an OSCC-patient-derived xenograft (OSCC-PDX) model. Methods: BALB/c nude mice with OSCC-PDX models were divided into Control, without PBMT (n = 8); Immediate irradiation, PBMT since one week after tumor implantation (n = 6); and Late irradiation, PBMT after tumors reached 200 mm3 (n = 6). OSCC-PDX were daily irradiated (660 nm; 100 mW; 6 J/cm2; 0,2 J/point) for 12 weeks. The tumors were collected and submitted to volumetric, histological, immunohistochemistry, and cell cycle analysis. Results: No significant differences in the volumetric measurements (p = 0.89) and in the histopathological grade (p > 0.05) were detected between the groups. The immunohistochemical analysis of Ki-67 (p = 0.9661); H3K9ac (p = 0.3794); and BMI1 (p = 0.5182), and the evaluation of the cell cycle phases (p > 0.05) by flow cytometry also did not demonstrate significant differences between the irradiated and non-irradiated groups. Conclusion: In this study, PBMT did not impact the behavior of OSCC-PDX models. This is an important preclinical outcome regarding safety concerns of the use of PBMT in cancer patients.",

keywords = "low-level light therapy, oral mucositis, oral squamous cell carcinoma, photobiomodulation therapy",

author = "Silveira, \{Felipe Martins\} and Schmidt, \{Tuany Rafaeli\} and Bruna Neumann and Cl{\'e}via Rosset and Zanella, \{Virgilio Gonzales\} and Maahs, \{Gerson Schulz\} and Martins, \{Marco Antonio Trevizani\} and Praveen Arany and Wagner, \{Vivian Petersen\} and Lopes, \{M{\'a}rcio Ajudarte\} and Santos-Silva, \{Alan Roger\} and Martins, \{Manoela Domingues\}",

note = "Publisher Copyright: {\textcopyright} 2021 Wiley Periodicals LLC.",

year = "2023",

month = mar,

doi = "10.1111/odi.13967",

language = "English",

volume = "29",

pages = "547--556",

journal = "Oral Diseases",

issn = "1354-523X",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Impact of photobiomodulation in a patient-derived xenograft model of oral squamous cell carcinoma

AU - Silveira, Felipe Martins

AU - Schmidt, Tuany Rafaeli

AU - Neumann, Bruna

AU - Rosset, Clévia

AU - Zanella, Virgilio Gonzales

AU - Maahs, Gerson Schulz

AU - Martins, Marco Antonio Trevizani

AU - Arany, Praveen

AU - Wagner, Vivian Petersen

AU - Lopes, Márcio Ajudarte

AU - Santos-Silva, Alan Roger

AU - Martins, Manoela Domingues

PY - 2023/3

Y1 - 2023/3

N2 - Background: Photobiomodulation therapy (PBMT) is an effective method for the prevention of oral mucositis. However, the effects of PBMT on oral squamous cell carcinoma (OSCC) have not yet been fully elucidated. This study aimed to evaluate the impact of PBMT in an OSCC-patient-derived xenograft (OSCC-PDX) model. Methods: BALB/c nude mice with OSCC-PDX models were divided into Control, without PBMT (n = 8); Immediate irradiation, PBMT since one week after tumor implantation (n = 6); and Late irradiation, PBMT after tumors reached 200 mm3 (n = 6). OSCC-PDX were daily irradiated (660 nm; 100 mW; 6 J/cm2; 0,2 J/point) for 12 weeks. The tumors were collected and submitted to volumetric, histological, immunohistochemistry, and cell cycle analysis. Results: No significant differences in the volumetric measurements (p = 0.89) and in the histopathological grade (p > 0.05) were detected between the groups. The immunohistochemical analysis of Ki-67 (p = 0.9661); H3K9ac (p = 0.3794); and BMI1 (p = 0.5182), and the evaluation of the cell cycle phases (p > 0.05) by flow cytometry also did not demonstrate significant differences between the irradiated and non-irradiated groups. Conclusion: In this study, PBMT did not impact the behavior of OSCC-PDX models. This is an important preclinical outcome regarding safety concerns of the use of PBMT in cancer patients.

AB - Background: Photobiomodulation therapy (PBMT) is an effective method for the prevention of oral mucositis. However, the effects of PBMT on oral squamous cell carcinoma (OSCC) have not yet been fully elucidated. This study aimed to evaluate the impact of PBMT in an OSCC-patient-derived xenograft (OSCC-PDX) model. Methods: BALB/c nude mice with OSCC-PDX models were divided into Control, without PBMT (n = 8); Immediate irradiation, PBMT since one week after tumor implantation (n = 6); and Late irradiation, PBMT after tumors reached 200 mm3 (n = 6). OSCC-PDX were daily irradiated (660 nm; 100 mW; 6 J/cm2; 0,2 J/point) for 12 weeks. The tumors were collected and submitted to volumetric, histological, immunohistochemistry, and cell cycle analysis. Results: No significant differences in the volumetric measurements (p = 0.89) and in the histopathological grade (p > 0.05) were detected between the groups. The immunohistochemical analysis of Ki-67 (p = 0.9661); H3K9ac (p = 0.3794); and BMI1 (p = 0.5182), and the evaluation of the cell cycle phases (p > 0.05) by flow cytometry also did not demonstrate significant differences between the irradiated and non-irradiated groups. Conclusion: In this study, PBMT did not impact the behavior of OSCC-PDX models. This is an important preclinical outcome regarding safety concerns of the use of PBMT in cancer patients.

KW - low-level light therapy

KW - oral mucositis

KW - oral squamous cell carcinoma

KW - photobiomodulation therapy

UR - https://www.scopus.com/pages/publications/85111353264

U2 - 10.1111/odi.13967

DO - 10.1111/odi.13967

M3 - Article

C2 - 34273227

AN - SCOPUS:85111353264

SN - 1354-523X

VL - 29

SP - 547

EP - 556

JO - Oral Diseases

JF - Oral Diseases

IS - 2

ER -

Impact of photobiomodulation in a patient-derived xenograft model of oral squamous cell carcinoma

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