Immunoaffinity Chromatography of Human β-Hexosaminidase A

Georgirene D. Vladutiu; Patrick J. Carmody; Mario C. Rattazzi

doi:10.1080/00327487508061567

Immunoaffinity Chromatography of Human β-Hexosaminidase A

Georgirene D. Vladutiu
, Patrick J. Carmody
, Mario C. Rattazzi

Pediatrics

Women and Children's Hospital of Buffalo

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A highly specific method for the purification of human β-hexos-aminidase A employing immunoaffinity chromatography is described. Using an antiserum against the unique antigenic determinant, a, of β-hexosaminidase A, and elution with 8.0M urea, a 283-or 417-fold purification of the enzyme was obtained in a single step from crude human placental homogenate.

Original language	English
Pages (from-to)	147-159
Number of pages	13
Journal	Preparative Biochemistry
Volume	5
Issue number	2
DOIs	https://doi.org/10.1080/00327487508061567
State	Published - Jan 1 1975

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title = "Immunoaffinity Chromatography of Human β-Hexosaminidase A",

abstract = "A highly specific method for the purification of human β-hexos-aminidase A employing immunoaffinity chromatography is described. Using an antiserum against the unique antigenic determinant, a, of β-hexosaminidase A, and elution with 8.0M urea, a 283-or 417-fold purification of the enzyme was obtained in a single step from crude human placental homogenate.",

author = "Vladutiu, \{Georgirene D.\} and Carmody, \{Patrick J.\} and Rattazzi, \{Mario C.\}",

year = "1975",

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AU - Vladutiu, Georgirene D.

AU - Carmody, Patrick J.

AU - Rattazzi, Mario C.

PY - 1975/1/1

Y1 - 1975/1/1

N2 - A highly specific method for the purification of human β-hexos-aminidase A employing immunoaffinity chromatography is described. Using an antiserum against the unique antigenic determinant, a, of β-hexosaminidase A, and elution with 8.0M urea, a 283-or 417-fold purification of the enzyme was obtained in a single step from crude human placental homogenate.

AB - A highly specific method for the purification of human β-hexos-aminidase A employing immunoaffinity chromatography is described. Using an antiserum against the unique antigenic determinant, a, of β-hexosaminidase A, and elution with 8.0M urea, a 283-or 417-fold purification of the enzyme was obtained in a single step from crude human placental homogenate.

UR - https://www.scopus.com/pages/publications/0016681822

U2 - 10.1080/00327487508061567

DO - 10.1080/00327487508061567

M3 - Article

C2 - 49891

AN - SCOPUS:0016681822

SN - 0032-7484

VL - 5

SP - 147

EP - 159

JO - Preparative Biochemistry

JF - Preparative Biochemistry

IS - 2

ER -

Immunoaffinity Chromatography of Human β-Hexosaminidase A

Abstract

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