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Immune response to moraxella catarrhalis in children with otitis media: Opsonophagocytosis with antigen-coated latex beads

  • SUNY Buffalo

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Opsonic antibody activity against Moraxella catarrhalis was determined in sera from children with otitis media. The antibody was determined with a new assay utilizing outer membrane antigen-coated latex beads. Antigen-coated beads opsonized in heat-inactivated pooled human serum phagocytosed 47.5 ± 36.1 beads per 100 neutrophils compared to 15.6 ± 10.2 beads per 100 neutrophils opsonized in hypogammaglobulinemic serum (p < .025). Antigen-coated beads opsonized in homologous sera from 11 children with M catarrhalis otitis media demonstrated increased opsonic activity in convalescent sera (34.6 ± 27.1) compared to acute sera (15.5 ± 6.7;p< .05). These data suggest that infection with M catarrhalis is associated with the development of opsonic antibody.

Original languageEnglish
Pages (from-to)522-524
Number of pages3
JournalAnnals of Otology, Rhinology and Laryngology
Volume103
Issue number7
DOIs
StatePublished - Jul 1994

Keywords

  • Moraxella Catarrhalis
  • opsonic antibody
  • otitis media

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