Identification of metabolites of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) formed on incubation with isolated rat hepatocytes

Tadashi Sawahata; James R. Olson; Robert A. Neal

doi:10.1016/S0006-291X(82)80050-8

Identification of metabolites of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) formed on incubation with isolated rat hepatocytes

Tadashi Sawahata
, James R. Olson
, Robert A. Neal

Department of Epidemiology and Environmental Health

CIIT Centers for Health Research

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Tritiated TCDD was incubated with isolated rat hepatocytes and the resulting incubation mixture subjected to high performance liquid chromatography (HPLC). The major peak of radioactivity not corresponding to TCDD was incubated with β-glucuronidase. The products of this reaction were derivatized with diazomethane and again subjected to HPLC. Two of the radioactive metabolites isolated by this procedure were found to co-migrate with authentic 1-methoxy-2,3,7,8-tetrachlorodibenzo-p-dioxin and 8-methoxy-2,3,7-trichlorodibenzo-p-dioxin. The identity of these metabolites was also confirmed by gas chromatography-mass spectrometry.

Original language	English
Pages (from-to)	341-346
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(82)80050-8
State	Published - Mar 15 1982

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title = "Identification of metabolites of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) formed on incubation with isolated rat hepatocytes",

abstract = "Tritiated TCDD was incubated with isolated rat hepatocytes and the resulting incubation mixture subjected to high performance liquid chromatography (HPLC). The major peak of radioactivity not corresponding to TCDD was incubated with β-glucuronidase. The products of this reaction were derivatized with diazomethane and again subjected to HPLC. Two of the radioactive metabolites isolated by this procedure were found to co-migrate with authentic 1-methoxy-2,3,7,8-tetrachlorodibenzo-p-dioxin and 8-methoxy-2,3,7-trichlorodibenzo-p-dioxin. The identity of these metabolites was also confirmed by gas chromatography-mass spectrometry.",

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T1 - Identification of metabolites of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) formed on incubation with isolated rat hepatocytes

AU - Sawahata, Tadashi

AU - Olson, James R.

AU - Neal, Robert A.

PY - 1982/3/15

Y1 - 1982/3/15

N2 - Tritiated TCDD was incubated with isolated rat hepatocytes and the resulting incubation mixture subjected to high performance liquid chromatography (HPLC). The major peak of radioactivity not corresponding to TCDD was incubated with β-glucuronidase. The products of this reaction were derivatized with diazomethane and again subjected to HPLC. Two of the radioactive metabolites isolated by this procedure were found to co-migrate with authentic 1-methoxy-2,3,7,8-tetrachlorodibenzo-p-dioxin and 8-methoxy-2,3,7-trichlorodibenzo-p-dioxin. The identity of these metabolites was also confirmed by gas chromatography-mass spectrometry.

AB - Tritiated TCDD was incubated with isolated rat hepatocytes and the resulting incubation mixture subjected to high performance liquid chromatography (HPLC). The major peak of radioactivity not corresponding to TCDD was incubated with β-glucuronidase. The products of this reaction were derivatized with diazomethane and again subjected to HPLC. Two of the radioactive metabolites isolated by this procedure were found to co-migrate with authentic 1-methoxy-2,3,7,8-tetrachlorodibenzo-p-dioxin and 8-methoxy-2,3,7-trichlorodibenzo-p-dioxin. The identity of these metabolites was also confirmed by gas chromatography-mass spectrometry.

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DO - 10.1016/S0006-291X(82)80050-8

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AN - SCOPUS:0020409470

SN - 0006-291X

VL - 105

SP - 341

EP - 346

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Identification of metabolites of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) formed on incubation with isolated rat hepatocytes

Abstract

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