@inproceedings{ea456630b8db491cb3f031145fa3af98,
title = "Hyperbranched polysiloxysilane nanoparticles for nonviral gene delivery vectors and nanoprobes",
abstract = "We report an approach to produce predefined surface charge tunable gene delivery vectors using siloxysilsane-based polymer for gene delivery studies. To obtain nonviral vectors, new series of hyperbranched polysiloxysilane (HBPS) were synthesized, and the end groups in polymer structures have modified with hydrophilic molecules; in other words, carboxylic acid and quaternary ammonium groups were employed into terminal structures to give the amphiphilicity. The novelty of these amphiphilic HBPS polymers lies in the fact that nanoparticles with different zeta potential (surface charge density) can be easily tailored and functionalized. These polymeric nanoparticles which containing various chemical groups on the surface indicated altered surface charge distributions (from -40 to +64mV). Finally, the use of these nanoparticles as efficient gene delivery vectors was demonstrated by means of in vitro transfection study using β- galactosidase plasmid and pEGFP-N1 plasmid, and the most efficient combination was obtained using HBPS-CN30:70.",
keywords = "Hyperbranched polysiloxysilane, Multi-photon cellular imaging., Nanoprobe, Nonviral gene delivery vector, Polymer nanoparticle, Surface charge",
author = "Kim, \{Won Jin\} and Bonoiu, \{Adela C.\} and Lee, \{Kwang Sup\} and Teruaki Hayakawa and Cheng Xia and Kakimoto, \{Masa Aki\} and Pudavar, \{Haridas E.\} and Prasad, \{Paras N.\}",
year = "2009",
doi = "10.1117/12.824518",
language = "English",
isbn = "9780819476937",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Nanobiosystems",
note = "Nanobiosystems: Processing, Characterization, and Applications II ; Conference date: 05-08-2009 Through 06-08-2009",
}