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High‐performance liquid chromatographic assay of sulfapyridine and acetylsulfapyridine in biological fluids

  • Joel Owerbach
  • , Noreen F. Johnson
  • , Theodore R. Bates
  • , Henry J. Pieniaszek
  • , William J. Jusko
  • SUNY Buffalo

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A high‐pressure liquid chromatographic method for the sensitive, rapid, and specific determination of sulfapyridine and its N‐acetyl derivative in plasma and saliva was developed. A cyano‐bonded, reversed‐phase, high efficiency column was used. The system detected these sulfonamides in serum to 0.25 mg/liter and within only 6 min. Sulfapyridine was separated from its acetyl derivative with little interference from other drugs. The assay reproducibility was with 3%. The assay was highly useful for routine monitoring of patients receiving sulfasalazine for inflammatory bowel disease.

Original languageEnglish
Pages (from-to)1250-1253
Number of pages4
JournalJournal of Pharmaceutical Sciences
Volume67
Issue number9
DOIs
StatePublished - Sep 1978

Keywords

  • Dermatitis suppressants—sulfapyridine and acetyl metabolite, high‐performance liquid chromatographic analyses in biological fluids
  • High‐performance liquid. chromatography—analyses, sulfapyridine and acetyl metabolite in biological fluids
  • Sulfapyridine and acetyl metabolite—high‐performance liquid chromatographic analyses in biological fluids

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