Abstract
A high‐performance liquid chromatographic method is presented for the simultaneous determination of imipramine, desipramine, and their 2‐hydroxylated metabolites in plasma. The method involves a simple plasma extraction at basic pH with organic solvent, chromatography on a silica gel column, and fluorescence detection. Correlation with a GLC‐mass spectrometric method for imipramine and desipramine is illustrated. The method can detect 1 ng of each component/ml of plasma, sufficient sensitivity for pharmacokinetic studies.
| Original language | English |
|---|---|
| Pages (from-to) | 703-705 |
| Number of pages | 3 |
| Journal | Journal of Pharmaceutical Sciences |
| Volume | 68 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1979 |
Keywords
- Antidepressants, tricyclic—imipramine, desipramine, high‐performance liquid chromatographic analysis in plasma
- Desipramine—analysis in plasma, high‐performance liquid chromatography
- High‐performance liquid chromatography—analysis, imipramine, desipramine, 2‐hydroxylated metabolites in plasma
- Imipramine—analysis in plasma, high‐performance liquid chromatography, metabolites
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