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High resolution functional photoacoustic computed tomography of the mouse brain during electrical stimulation

  • Mohammad R.N. Avanaki
  • , Jun Xia
  • , Lihong V. Wang
  • Washington University St. Louis

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

16 Scopus citations

Abstract

Photoacoustic computed tomography (PACT) is an emerging imaging technique which is based on the acoustic detection of optical absorption from tissue chromophores, such as oxy-hemoglobin and deoxy-hemoglobin. An important application of PACT is functional brain imaging of small animals. The conversion of light to acoustic waves allows PACT to provide high resolution images of cortical vasculatures through the intact scalp. Here, PACT was utilized to study the activated areas of the mouse brain during forepaw and hindpaw stimulations. Temporal PACT images were acquired enabling computation of hemodynamic changes during stimulation. The stimulations were performed by trains of pulses at different stimulation currents (between 0.1 to 2 mA) and pulse repetition rates (between 0.05 Hz to 0.01Hz). The response at somatosensory cortex-forelimb, and somatosensory cortex-hindlimb, were investigated. The Paxinos mouse brain atlas was used to confirm the activated regions. The study shows that PACT is a promising new technology that can be used to study brain functionality with high spatial resolution.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2013
PublisherSPIE
ISBN (Print)9780819493507
DOIs
StatePublished - Mar 4 2013
EventPhotons Plus Ultrasound: Imaging and Sensing 2013 - San Francisco, CA, United States
Duration: Feb 3 2013Feb 5 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8581
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2013
Country/TerritoryUnited States
CitySan Francisco, CA
Period02/3/1302/5/13

Keywords

  • Electrical Stimulation
  • Functional Imaging
  • Photoacoustic Tomography

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