Hepatic DNA adduct formation in rats treated with benzo[f]quinoline

H. C. Sikka; C. Kandaswami; S. Kumar; J. P. Rutkowski; S. K. Dubey; K. Earley; R. C. Gupta

doi:10.1016/0304-3835(88)90225-X

Hepatic DNA adduct formation in rats treated with benzo[f]quinoline

H. C. Sikka
, C. Kandaswami
, S. Kumar
, J. P. Rutkowski
, S. K. Dubey
, K. Earley
, R. C. Gupta

Pharmacology and Toxicology

State University of New York System
Baylor College of Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The formation of hepatic DNA adducts in male Sprague-Dawley rats following i.p. administration of benzo[f]quinoline (BfQ) was examined using a ³²P-post-labeling assay. BfQ exhibited a low binding (11-27 amol adducts/μg DNA) to liver DNA. Two BfQ-nucleoside adducts (one major and one minor) were detected. The BfQ-DNA adducts formed in vivo were chromatographically distinct from the adducts formed by the reaction of calf thymus DNA in vitro with BfQ-5,6-oxide, syn-7β, 8α-dihydroxy-9β, 10β-epoxy-7,8,9,10-tetrahydroBfQ, anti-9α, 10β-dihydroxy-7α, 8α-epoxy-7,8,9,10-tetrahydroBfQ, or anti-7β, 8α-dihydroxy-9α, 10α-epoxy-7,8,9,10-tetrahydroBfQ-N-oxide. These results suggest that the bay-region diol epoxide of BfQ, unlike the bay-region diol epoxide derivatives of polynuclear aromatic hydrocarbons, is not involved in the covalent binding of BfQ to DNA.

Original language	English
Pages (from-to)	133-138
Number of pages	6
Journal	Cancer Letters
Volume	43
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3835(88)90225-X
State	Published - Dec 1 1988

Keywords

aza-polynuclear aromatic hydrocarbons
benzo[f]quinoline
DNA adducts, bay-region diol epoxide

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10.1016/0304-3835(88)90225-X

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title = "Hepatic DNA adduct formation in rats treated with benzo[f]quinoline",

abstract = "The formation of hepatic DNA adducts in male Sprague-Dawley rats following i.p. administration of benzo[f]quinoline (BfQ) was examined using a 32P-post-labeling assay. BfQ exhibited a low binding (11-27 amol adducts/μg DNA) to liver DNA. Two BfQ-nucleoside adducts (one major and one minor) were detected. The BfQ-DNA adducts formed in vivo were chromatographically distinct from the adducts formed by the reaction of calf thymus DNA in vitro with BfQ-5,6-oxide, syn-7β, 8α-dihydroxy-9β, 10β-epoxy-7,8,9,10-tetrahydroBfQ, anti-9α, 10β-dihydroxy-7α, 8α-epoxy-7,8,9,10-tetrahydroBfQ, or anti-7β, 8α-dihydroxy-9α, 10α-epoxy-7,8,9,10-tetrahydroBfQ-N-oxide. These results suggest that the bay-region diol epoxide of BfQ, unlike the bay-region diol epoxide derivatives of polynuclear aromatic hydrocarbons, is not involved in the covalent binding of BfQ to DNA.",

keywords = "aza-polynuclear aromatic hydrocarbons, benzo[f]quinoline, DNA adducts, bay-region diol epoxide",

author = "Sikka, \{H. C.\} and C. Kandaswami and S. Kumar and Rutkowski, \{J. P.\} and Dubey, \{S. K.\} and K. Earley and Gupta, \{R. C.\}",

year = "1988",

month = dec,

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doi = "10.1016/0304-3835(88)90225-X",

language = "English",

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T1 - Hepatic DNA adduct formation in rats treated with benzo[f]quinoline

AU - Sikka, H. C.

AU - Kandaswami, C.

AU - Kumar, S.

AU - Rutkowski, J. P.

AU - Dubey, S. K.

AU - Earley, K.

AU - Gupta, R. C.

PY - 1988/12/1

Y1 - 1988/12/1

N2 - The formation of hepatic DNA adducts in male Sprague-Dawley rats following i.p. administration of benzo[f]quinoline (BfQ) was examined using a 32P-post-labeling assay. BfQ exhibited a low binding (11-27 amol adducts/μg DNA) to liver DNA. Two BfQ-nucleoside adducts (one major and one minor) were detected. The BfQ-DNA adducts formed in vivo were chromatographically distinct from the adducts formed by the reaction of calf thymus DNA in vitro with BfQ-5,6-oxide, syn-7β, 8α-dihydroxy-9β, 10β-epoxy-7,8,9,10-tetrahydroBfQ, anti-9α, 10β-dihydroxy-7α, 8α-epoxy-7,8,9,10-tetrahydroBfQ, or anti-7β, 8α-dihydroxy-9α, 10α-epoxy-7,8,9,10-tetrahydroBfQ-N-oxide. These results suggest that the bay-region diol epoxide of BfQ, unlike the bay-region diol epoxide derivatives of polynuclear aromatic hydrocarbons, is not involved in the covalent binding of BfQ to DNA.

AB - The formation of hepatic DNA adducts in male Sprague-Dawley rats following i.p. administration of benzo[f]quinoline (BfQ) was examined using a 32P-post-labeling assay. BfQ exhibited a low binding (11-27 amol adducts/μg DNA) to liver DNA. Two BfQ-nucleoside adducts (one major and one minor) were detected. The BfQ-DNA adducts formed in vivo were chromatographically distinct from the adducts formed by the reaction of calf thymus DNA in vitro with BfQ-5,6-oxide, syn-7β, 8α-dihydroxy-9β, 10β-epoxy-7,8,9,10-tetrahydroBfQ, anti-9α, 10β-dihydroxy-7α, 8α-epoxy-7,8,9,10-tetrahydroBfQ, or anti-7β, 8α-dihydroxy-9α, 10α-epoxy-7,8,9,10-tetrahydroBfQ-N-oxide. These results suggest that the bay-region diol epoxide of BfQ, unlike the bay-region diol epoxide derivatives of polynuclear aromatic hydrocarbons, is not involved in the covalent binding of BfQ to DNA.

KW - aza-polynuclear aromatic hydrocarbons

KW - benzo[f]quinoline

KW - DNA adducts, bay-region diol epoxide

UR - https://www.scopus.com/pages/publications/0024207527

U2 - 10.1016/0304-3835(88)90225-X

DO - 10.1016/0304-3835(88)90225-X

M3 - Article

C2 - 3203322

AN - SCOPUS:0024207527

SN - 0304-3835

VL - 43

SP - 133

EP - 138

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

Hepatic DNA adduct formation in rats treated with benzo[f]quinoline

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Keywords

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