GFT projection NMR for efficient 1H/13C sugar spin system identification in nucleic acids

Hanudatta S. Atreya; Bharathwaj Sathyamoorthy; Garima Jaipuria; Victor Beaumont; Gabriele Varani; Thomas Szyperski

doi:10.1007/s10858-012-9687-5

GFT projection NMR for efficient ¹H/¹³C sugar spin system identification in nucleic acids

Hanudatta S. Atreya
, Bharathwaj Sathyamoorthy
, Garima Jaipuria
, Victor Beaumont
, Gabriele Varani
, Thomas Szyperski

Chemistry

Indian Institute of Science Bangalore
SUNY Buffalo
University of Washington

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify ¹H/¹³C sugar spin systems in ¹³C labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of ¹³C-¹H groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1′- and 3′-CH signal dispersion for complete spin system identification including 5′-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3′-untranslated region of the pre-mRNA of human U1A protein.

Original language	English
Pages (from-to)	337-342
Number of pages	6
Journal	Journal of Biomolecular NMR
Volume	54
Issue number	4
DOIs	https://doi.org/10.1007/s10858-012-9687-5
State	Published - Dec 2012

Keywords

GFT projection NMR
Nucleic acids
Resonance assignment
RNA

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10.1007/s10858-012-9687-5

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title = "GFT projection NMR for efficient 1H/13C sugar spin system identification in nucleic acids",

abstract = "A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify 1H/13C sugar spin systems in 13C labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of 13C-1H groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1′- and 3′-CH signal dispersion for complete spin system identification including 5′-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3′-untranslated region of the pre-mRNA of human U1A protein.",

keywords = "GFT projection NMR, Nucleic acids, Resonance assignment, RNA",

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year = "2012",

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doi = "10.1007/s10858-012-9687-5",

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T1 - GFT projection NMR for efficient 1H/13C sugar spin system identification in nucleic acids

AU - Atreya, Hanudatta S.

AU - Sathyamoorthy, Bharathwaj

AU - Jaipuria, Garima

AU - Beaumont, Victor

AU - Varani, Gabriele

AU - Szyperski, Thomas

PY - 2012/12

Y1 - 2012/12

N2 - A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify 1H/13C sugar spin systems in 13C labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of 13C-1H groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1′- and 3′-CH signal dispersion for complete spin system identification including 5′-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3′-untranslated region of the pre-mRNA of human U1A protein.

AB - A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify 1H/13C sugar spin systems in 13C labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of 13C-1H groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1′- and 3′-CH signal dispersion for complete spin system identification including 5′-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3′-untranslated region of the pre-mRNA of human U1A protein.

KW - GFT projection NMR

KW - Nucleic acids

KW - Resonance assignment

KW - RNA

UR - https://www.scopus.com/pages/publications/84874453966

U2 - 10.1007/s10858-012-9687-5

DO - 10.1007/s10858-012-9687-5

M3 - Article

C2 - 23192291

AN - SCOPUS:84874453966

SN - 0925-2738

VL - 54

SP - 337

EP - 342

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 4

ER -

GFT projection NMR for efficient ¹H/¹³C sugar spin system identification in nucleic acids

Abstract

Keywords

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