Genomic instability of human aberrant crypt foci measured by inter-(simple sequence repeat) PCR and array-CGH

Sadir J. Alrawi; Robert E. Carroll; Hank C. Hill; John F. Gibbs; Dongfeng Tan; Bruce M. Brenner; Norma J. Nowak; Helen Swede; Daniel L. Stoler; Garth R. Anderson

doi:10.1016/j.mrfmmm.2006.05.022

Genomic instability of human aberrant crypt foci measured by inter-(simple sequence repeat) PCR and array-CGH

Sadir J. Alrawi
, Robert E. Carroll
, Hank C. Hill
, John F. Gibbs
, Dongfeng Tan
, Bruce M. Brenner
, Norma J. Nowak
, Helen Swede
, Daniel L. Stoler
, Garth R. Anderson

Biochemistry

Roswell Park Cancer Institute
University of Illinois at Chicago
Medical College of Wisconsin
University of Connecticut

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Aberrant crypt foci (ACF) are the earliest identifiable neoplastic lesions in the colon. Thirty-two ACFs were examined for genomic instability in forms detectable either by inter-(simple sequence repeat) PCR or by array comparative genomic hybridization [array-CGH]. One-fourth of ACFs revealed moderate instability by inter-(simple sequence repeat) PCR; none showed amplifications or deletions on array-CGH. The absence of genomic events detectible by BAC array-CGH indicates early events in colorectal tumor progression are typically smaller than the approximate 150 kb size of a BAC clone insert.

Original language	English
Pages (from-to)	30-38
Number of pages	9
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	601
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mrfmmm.2006.05.022
State	Published - Oct 10 2006

Keywords

Aberrant crypt foci
Colorectal cancer
Genomic instability

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10.1016/j.mrfmmm.2006.05.022

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Alrawi, S. J., Carroll, R. E., Hill, H. C., Gibbs, J. F., Tan, D., Brenner, B. M., Nowak, N. J., Swede, H., Stoler, D. L., & Anderson, G. R. (2006). Genomic instability of human aberrant crypt foci measured by inter-(simple sequence repeat) PCR and array-CGH. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 601(1-2), 30-38. https://doi.org/10.1016/j.mrfmmm.2006.05.022

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title = "Genomic instability of human aberrant crypt foci measured by inter-(simple sequence repeat) PCR and array-CGH",

abstract = "Aberrant crypt foci (ACF) are the earliest identifiable neoplastic lesions in the colon. Thirty-two ACFs were examined for genomic instability in forms detectable either by inter-(simple sequence repeat) PCR or by array comparative genomic hybridization [array-CGH]. One-fourth of ACFs revealed moderate instability by inter-(simple sequence repeat) PCR; none showed amplifications or deletions on array-CGH. The absence of genomic events detectible by BAC array-CGH indicates early events in colorectal tumor progression are typically smaller than the approximate 150 kb size of a BAC clone insert.",

keywords = "Aberrant crypt foci, Colorectal cancer, Genomic instability",

author = "Alrawi, \{Sadir J.\} and Carroll, \{Robert E.\} and Hill, \{Hank C.\} and Gibbs, \{John F.\} and Dongfeng Tan and Brenner, \{Bruce M.\} and Nowak, \{Norma J.\} and Helen Swede and Stoler, \{Daniel L.\} and Anderson, \{Garth R.\}",

year = "2006",

month = oct,

day = "10",

doi = "10.1016/j.mrfmmm.2006.05.022",

language = "English",

volume = "601",

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journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",

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Alrawi, SJ, Carroll, RE, Hill, HC, Gibbs, JF, Tan, D, Brenner, BM, Nowak, NJ, Swede, H, Stoler, DL & Anderson, GR 2006, 'Genomic instability of human aberrant crypt foci measured by inter-(simple sequence repeat) PCR and array-CGH', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol. 601, no. 1-2, pp. 30-38. https://doi.org/10.1016/j.mrfmmm.2006.05.022

TY - JOUR

T1 - Genomic instability of human aberrant crypt foci measured by inter-(simple sequence repeat) PCR and array-CGH

AU - Alrawi, Sadir J.

AU - Carroll, Robert E.

AU - Hill, Hank C.

AU - Gibbs, John F.

AU - Tan, Dongfeng

AU - Brenner, Bruce M.

AU - Nowak, Norma J.

AU - Swede, Helen

AU - Stoler, Daniel L.

AU - Anderson, Garth R.

PY - 2006/10/10

Y1 - 2006/10/10

N2 - Aberrant crypt foci (ACF) are the earliest identifiable neoplastic lesions in the colon. Thirty-two ACFs were examined for genomic instability in forms detectable either by inter-(simple sequence repeat) PCR or by array comparative genomic hybridization [array-CGH]. One-fourth of ACFs revealed moderate instability by inter-(simple sequence repeat) PCR; none showed amplifications or deletions on array-CGH. The absence of genomic events detectible by BAC array-CGH indicates early events in colorectal tumor progression are typically smaller than the approximate 150 kb size of a BAC clone insert.

AB - Aberrant crypt foci (ACF) are the earliest identifiable neoplastic lesions in the colon. Thirty-two ACFs were examined for genomic instability in forms detectable either by inter-(simple sequence repeat) PCR or by array comparative genomic hybridization [array-CGH]. One-fourth of ACFs revealed moderate instability by inter-(simple sequence repeat) PCR; none showed amplifications or deletions on array-CGH. The absence of genomic events detectible by BAC array-CGH indicates early events in colorectal tumor progression are typically smaller than the approximate 150 kb size of a BAC clone insert.

KW - Aberrant crypt foci

KW - Colorectal cancer

KW - Genomic instability

UR - https://www.scopus.com/pages/publications/33749511582

U2 - 10.1016/j.mrfmmm.2006.05.022

DO - 10.1016/j.mrfmmm.2006.05.022

M3 - Article

C2 - 16806294

AN - SCOPUS:33749511582

SN - 1386-1964

VL - 601

SP - 30

EP - 38

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

IS - 1-2

ER -

Genomic instability of human aberrant crypt foci measured by inter-(simple sequence repeat) PCR and array-CGH

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Keywords

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