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Genomic DNA of leukemic patients: A molecular tool to detect genetic variation in clinical diagnosis

  • Munmun Rawat
  • , Sanjeev Malhotra
  • , C. S. Senthilkumar
  • , Ujjwala Kulkarni
  • , N. Ganesh
  • Jawaharlal Nehru Cancer Hospital and Research Centre

Research output: Contribution to journalArticlepeer-review

Abstract

A Comparative study analyzed to detect the genetic variation in CML, AML, CLL and ALL leukemic patients. DNA was isolated from four leukemia patient's blood sample and electrophoresed in 0.8% agarose gel. Purity was checked by quantification at 260 nm in UV - spectrophotometer. In all the four leukemia cases, CML patient DNA was significantly noted with elevated level. CLL patient DNA samples also found with slight variation in increased concentration. AML and ALL samples showed no variation on their comparative status. The present study concluded all the elevated level was found in chronic leukemia as compared to acute leukemia's.

Original languageEnglish
Pages (from-to)745-748
Number of pages4
JournalBiosciences Biotechnology Research Asia
Volume4
Issue number2
StatePublished - Dec 2007

Keywords

  • Comparative studies
  • Genetic variation
  • Genomic DNA
  • Leukemia's

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