@inbook{67e45caba6784d4fb88a1bd305624db8,
title = "Generating Monocyte-Derived Endothelial-like Cells for Vascular Regeneration",
abstract = "A major limitation in engineering vascular grafts is the lack of proper endothelium to prevent thrombosis and subsequent graft failure. Obtaining endothelial cells from patients{\textquoteright} vasculature is intrusive and requires extensive culture time. Here we present an alternative strategy wherein abundant and easily accessible monocytes from peripheral blood are cultured and differentiated towards an endothelial-like state capable of preventing thrombosis through production of nitric oxide and formation of endothelial adherens junctions. Considering the plethora of monocytes present within peripheral blood, this method provides a robust alternative to generating endothelial cells required for vascular graft production.",
keywords = "Endothelial cells, Fibronectin, Monocytes, Platelet-rich plasma, Shear, VEGF",
author = "Smith, \{Randall J.\} and Andreadis, \{Stelios T.\}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1708-3\_2",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "13--19",
booktitle = "Methods in Molecular Biology",
}