Functions of conserved motifs in the RNA-dependent RNA polymerase of a yeast double-stranded RNA virus

At least eight conserved motifs are visible in the totivirus RNA- dependent RNA polymerase (RDRP). We have systematically altered each of these in the Saccharomyces cerevisiae double-stranded RNA virus ScVL1 by substituting the conserved motifs from a giardiavirus. The results help define the conserved regions of the RDRP involved in polymerase function and those essential for other reasons.