Fiber-optic-based immunosensors for haptens

Thomas A. Betts; Gino C. Catena; Jingfan Huang; Kevin S. Litwiler; Jing Zhang; Jo Ann Zagrobelny; Frank V. Bright

doi:10.1016/S0003-2670(00)80664-9

Fiber-optic-based immunosensors for haptens

Thomas A. Betts
, Gino C. Catena
, Jingfan Huang
, Kevin S. Litwiler
, Jing Zhang
, Jo Ann Zagrobelny
, Frank V. Bright

Chemistry

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A selective fiber-optic-based immunosensor for haptens is described and characterized. In order to construct a sensor for different analytes, a prescribed reaction protocol is followed and only the recognition element [F_(ab′) antibody fragment] has to be changed. The dynamic range of this sensor is about two orders of magnitude and the detection limits are at least 25 nM (25-μ1 volume). By using steady-state fluorescence and classical ultra-high-vacuum surface analytical techniques, the effects of regeneration media, reaction and storage times and interferents on the sensor response were studied.

Original language	English
Pages (from-to)	55-63
Number of pages	9
Journal	Analytica Chimica Acta
Volume	246
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2670(00)80664-9
State	Published - May 15 1991

Keywords

Fibre-optic sensor
Fluorimetry
Haptens
Immunosensors

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10.1016/S0003-2670(00)80664-9

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title = "Fiber-optic-based immunosensors for haptens",

abstract = "A selective fiber-optic-based immunosensor for haptens is described and characterized. In order to construct a sensor for different analytes, a prescribed reaction protocol is followed and only the recognition element [F(ab′) antibody fragment] has to be changed. The dynamic range of this sensor is about two orders of magnitude and the detection limits are at least 25 nM (25-μ1 volume). By using steady-state fluorescence and classical ultra-high-vacuum surface analytical techniques, the effects of regeneration media, reaction and storage times and interferents on the sensor response were studied.",

keywords = "Fibre-optic sensor, Fluorimetry, Haptens, Immunosensors",

author = "Betts, \{Thomas A.\} and Catena, \{Gino C.\} and Jingfan Huang and Litwiler, \{Kevin S.\} and Jing Zhang and Zagrobelny, \{Jo Ann\} and Bright, \{Frank V.\}",

year = "1991",

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doi = "10.1016/S0003-2670(00)80664-9",

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AU - Betts, Thomas A.

AU - Catena, Gino C.

AU - Huang, Jingfan

AU - Litwiler, Kevin S.

AU - Zhang, Jing

AU - Zagrobelny, Jo Ann

AU - Bright, Frank V.

PY - 1991/5/15

Y1 - 1991/5/15

N2 - A selective fiber-optic-based immunosensor for haptens is described and characterized. In order to construct a sensor for different analytes, a prescribed reaction protocol is followed and only the recognition element [F(ab′) antibody fragment] has to be changed. The dynamic range of this sensor is about two orders of magnitude and the detection limits are at least 25 nM (25-μ1 volume). By using steady-state fluorescence and classical ultra-high-vacuum surface analytical techniques, the effects of regeneration media, reaction and storage times and interferents on the sensor response were studied.

AB - A selective fiber-optic-based immunosensor for haptens is described and characterized. In order to construct a sensor for different analytes, a prescribed reaction protocol is followed and only the recognition element [F(ab′) antibody fragment] has to be changed. The dynamic range of this sensor is about two orders of magnitude and the detection limits are at least 25 nM (25-μ1 volume). By using steady-state fluorescence and classical ultra-high-vacuum surface analytical techniques, the effects of regeneration media, reaction and storage times and interferents on the sensor response were studied.

KW - Fibre-optic sensor

KW - Fluorimetry

KW - Haptens

KW - Immunosensors

UR - https://www.scopus.com/pages/publications/0025892247

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VL - 246

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EP - 63

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1

ER -

Fiber-optic-based immunosensors for haptens

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Keywords

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