Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry

Aurelie Roux; Shelley N. Jackson; Ludovic Muller; Damon Barbacci; Joseph O'Rourke; Panayotis K. Thanos; Nora D. Volkow; Carey Balaban; J. Albert Schultz; Amina S. Woods

doi:10.1021/acschemneuro.6b00120

Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry

Aurelie Roux
, Shelley N. Jackson
, Ludovic Muller
, Damon Barbacci
, Joseph O'Rourke
, Panayotis K. Thanos
, Nora D. Volkow
, Carey Balaban
, J. Albert Schultz
, Amina S. Woods

Pharmacology and Toxicology

National Institutes of Health
University of Pittsburgh
Ionwerks Inc.
SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Alcohol abuse is a chronic disease characterized by the consumption of alcohol at a level that interferes with physical and mental health and causes serious and persistent changes in the brain. Lipid metabolism is of particular interest due to its high concentration in the brain. Lipids are the main component of cell membranes, are involved in cell signaling, signal transduction, and energy storage. In this study, we analyzed lipid composition of chronically ethanol exposed mouse brains. Juvenile (JUV) and adult (ADU) mice were placed on a daily limited-access ethanol intake model for 52 days. After euthanasia, brains were harvested, and total lipids were extracted from brain homogenates. Samples were analyzed using high resolution mass spectrometry and processed by multivariate and univariate statistical analysis. Significant lipid changes were observed in different classes including sphingolipids, fatty acids, lysophosphatidylcholines, and other glycerophospholipids.

Original language	English
Pages (from-to)	1148-1156
Number of pages	9
Journal	ACS Chemical Neuroscience
Volume	7
Issue number	8
DOIs	https://doi.org/10.1021/acschemneuro.6b00120
State	Published - Aug 17 2016

Keywords

alcohol
electrospray ionization
lipids
mass spectrometry
solid-phase extraction

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10.1021/acschemneuro.6b00120

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title = "Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry",

abstract = "Alcohol abuse is a chronic disease characterized by the consumption of alcohol at a level that interferes with physical and mental health and causes serious and persistent changes in the brain. Lipid metabolism is of particular interest due to its high concentration in the brain. Lipids are the main component of cell membranes, are involved in cell signaling, signal transduction, and energy storage. In this study, we analyzed lipid composition of chronically ethanol exposed mouse brains. Juvenile (JUV) and adult (ADU) mice were placed on a daily limited-access ethanol intake model for 52 days. After euthanasia, brains were harvested, and total lipids were extracted from brain homogenates. Samples were analyzed using high resolution mass spectrometry and processed by multivariate and univariate statistical analysis. Significant lipid changes were observed in different classes including sphingolipids, fatty acids, lysophosphatidylcholines, and other glycerophospholipids.",

keywords = "alcohol, electrospray ionization, lipids, mass spectrometry, solid-phase extraction",

author = "Aurelie Roux and Jackson, \{Shelley N.\} and Ludovic Muller and Damon Barbacci and Joseph O'Rourke and Thanos, \{Panayotis K.\} and Volkow, \{Nora D.\} and Carey Balaban and Schultz, \{J. Albert\} and Woods, \{Amina S.\}",

note = "Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

year = "2016",

month = aug,

day = "17",

doi = "10.1021/acschemneuro.6b00120",

language = "English",

volume = "7",

pages = "1148--1156",

journal = "ACS Chemical Neuroscience",

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TY - JOUR

T1 - Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry

AU - Roux, Aurelie

AU - Jackson, Shelley N.

AU - Muller, Ludovic

AU - Barbacci, Damon

AU - O'Rourke, Joseph

AU - Thanos, Panayotis K.

AU - Volkow, Nora D.

AU - Balaban, Carey

AU - Schultz, J. Albert

AU - Woods, Amina S.

PY - 2016/8/17

Y1 - 2016/8/17

N2 - Alcohol abuse is a chronic disease characterized by the consumption of alcohol at a level that interferes with physical and mental health and causes serious and persistent changes in the brain. Lipid metabolism is of particular interest due to its high concentration in the brain. Lipids are the main component of cell membranes, are involved in cell signaling, signal transduction, and energy storage. In this study, we analyzed lipid composition of chronically ethanol exposed mouse brains. Juvenile (JUV) and adult (ADU) mice were placed on a daily limited-access ethanol intake model for 52 days. After euthanasia, brains were harvested, and total lipids were extracted from brain homogenates. Samples were analyzed using high resolution mass spectrometry and processed by multivariate and univariate statistical analysis. Significant lipid changes were observed in different classes including sphingolipids, fatty acids, lysophosphatidylcholines, and other glycerophospholipids.

AB - Alcohol abuse is a chronic disease characterized by the consumption of alcohol at a level that interferes with physical and mental health and causes serious and persistent changes in the brain. Lipid metabolism is of particular interest due to its high concentration in the brain. Lipids are the main component of cell membranes, are involved in cell signaling, signal transduction, and energy storage. In this study, we analyzed lipid composition of chronically ethanol exposed mouse brains. Juvenile (JUV) and adult (ADU) mice were placed on a daily limited-access ethanol intake model for 52 days. After euthanasia, brains were harvested, and total lipids were extracted from brain homogenates. Samples were analyzed using high resolution mass spectrometry and processed by multivariate and univariate statistical analysis. Significant lipid changes were observed in different classes including sphingolipids, fatty acids, lysophosphatidylcholines, and other glycerophospholipids.

KW - alcohol

KW - electrospray ionization

KW - lipids

KW - mass spectrometry

KW - solid-phase extraction

UR - https://www.scopus.com/pages/publications/84983428658

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DO - 10.1021/acschemneuro.6b00120

M3 - Article

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AN - SCOPUS:84983428658

SN - 1948-7193

VL - 7

SP - 1148

EP - 1156

JO - ACS Chemical Neuroscience

JF - ACS Chemical Neuroscience

IS - 8

ER -

Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry

Abstract

Keywords

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