Estradiol-17β stimulates phosphate uptake and is mitogenic for primary rabbit renal proximal tubule cells

Ho Jae Han; Yeune Hee Lee; Kwon Moo Park; Mary Taub

doi:10.1159/000065300

Estradiol-17β stimulates phosphate uptake and is mitogenic for primary rabbit renal proximal tubule cells

Ho Jae Han
, Yeune Hee Lee
, Kwon Moo Park
, Mary Taub

Biochemistry

Chonnam National University

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The direct effects of estradiol-17β (E₂) on phosphate (P_i) uptake and on DNA synthesis in the primary rabbit kidney proximal tubule cells (PTCs) have been investigated. In the present study, E₂ (>10^-9 M, over 9 days) causes an increase both in [³H]thymidine incorporation and the number of PTCs. The anti-estrogen tamoxifen completely prevented the E₂-induced increase in [³H]thymidine incorporation, and ameliorated the stimulatory effect of E₂ on growth. E₂ (>10^-9 M, over 5 days also stimulated the P_i uptake and its effect was due to the V_max values but not to the K_m value for P_i uptake. Estriol and estrone also exerted significant stimulatory effects on P_i uptake. Progesterone, tamoxifen, actinomycin D and cycloheximide prevented the E₂-induced stimulation of P_i uptake. In conclusion, estrogens at physiological concentrations stimulate P_i uptake and DNA synthesis in the renal proximal tubule cells, and these effects are estrogen receptor mediated. Copyright

Original language	English
Pages (from-to)	355-364
Number of pages	10
Journal	Experimental Nephrology
Volume	10
Issue number	5-6
DOIs	https://doi.org/10.1159/000065300
State	Published - 2002

Keywords

Estradiol-17β
Kidney
Na/P cotransport
Phosphate

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10.1159/000065300

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@article{1f452747a316427c9250851d3964f276,

title = "Estradiol-17β stimulates phosphate uptake and is mitogenic for primary rabbit renal proximal tubule cells",

abstract = "The direct effects of estradiol-17β (E2) on phosphate (Pi) uptake and on DNA synthesis in the primary rabbit kidney proximal tubule cells (PTCs) have been investigated. In the present study, E2 (>10-9 M, over 9 days) causes an increase both in [3H]thymidine incorporation and the number of PTCs. The anti-estrogen tamoxifen completely prevented the E2-induced increase in [3H]thymidine incorporation, and ameliorated the stimulatory effect of E2 on growth. E2 (>10-9 M, over 5 days also stimulated the Pi uptake and its effect was due to the Vmax values but not to the Km value for Pi uptake. Estriol and estrone also exerted significant stimulatory effects on Pi uptake. Progesterone, tamoxifen, actinomycin D and cycloheximide prevented the E2-induced stimulation of Pi uptake. In conclusion, estrogens at physiological concentrations stimulate Pi uptake and DNA synthesis in the renal proximal tubule cells, and these effects are estrogen receptor mediated. Copyright",

keywords = "Estradiol-17β, Kidney, Na/P cotransport, Phosphate",

author = "Han, \{Ho Jae\} and Lee, \{Yeune Hee\} and Park, \{Kwon Moo\} and Mary Taub",

year = "2002",

doi = "10.1159/000065300",

language = "English",

volume = "10",

pages = "355--364",

journal = "Experimental Nephrology",

issn = "1018-7782",

publisher = "S. Karger AG",

number = "5-6",

}

TY - JOUR

T1 - Estradiol-17β stimulates phosphate uptake and is mitogenic for primary rabbit renal proximal tubule cells

AU - Han, Ho Jae

AU - Lee, Yeune Hee

AU - Park, Kwon Moo

AU - Taub, Mary

PY - 2002

Y1 - 2002

N2 - The direct effects of estradiol-17β (E2) on phosphate (Pi) uptake and on DNA synthesis in the primary rabbit kidney proximal tubule cells (PTCs) have been investigated. In the present study, E2 (>10-9 M, over 9 days) causes an increase both in [3H]thymidine incorporation and the number of PTCs. The anti-estrogen tamoxifen completely prevented the E2-induced increase in [3H]thymidine incorporation, and ameliorated the stimulatory effect of E2 on growth. E2 (>10-9 M, over 5 days also stimulated the Pi uptake and its effect was due to the Vmax values but not to the Km value for Pi uptake. Estriol and estrone also exerted significant stimulatory effects on Pi uptake. Progesterone, tamoxifen, actinomycin D and cycloheximide prevented the E2-induced stimulation of Pi uptake. In conclusion, estrogens at physiological concentrations stimulate Pi uptake and DNA synthesis in the renal proximal tubule cells, and these effects are estrogen receptor mediated. Copyright

AB - The direct effects of estradiol-17β (E2) on phosphate (Pi) uptake and on DNA synthesis in the primary rabbit kidney proximal tubule cells (PTCs) have been investigated. In the present study, E2 (>10-9 M, over 9 days) causes an increase both in [3H]thymidine incorporation and the number of PTCs. The anti-estrogen tamoxifen completely prevented the E2-induced increase in [3H]thymidine incorporation, and ameliorated the stimulatory effect of E2 on growth. E2 (>10-9 M, over 5 days also stimulated the Pi uptake and its effect was due to the Vmax values but not to the Km value for Pi uptake. Estriol and estrone also exerted significant stimulatory effects on Pi uptake. Progesterone, tamoxifen, actinomycin D and cycloheximide prevented the E2-induced stimulation of Pi uptake. In conclusion, estrogens at physiological concentrations stimulate Pi uptake and DNA synthesis in the renal proximal tubule cells, and these effects are estrogen receptor mediated. Copyright

KW - Estradiol-17β

KW - Kidney

KW - Na/P cotransport

KW - Phosphate

UR - https://www.scopus.com/pages/publications/0036882270

U2 - 10.1159/000065300

DO - 10.1159/000065300

M3 - Article

C2 - 12381920

AN - SCOPUS:0036882270

SN - 1018-7782

VL - 10

SP - 355

EP - 364

JO - Experimental Nephrology

JF - Experimental Nephrology

IS - 5-6

ER -

Estradiol-17β stimulates phosphate uptake and is mitogenic for primary rabbit renal proximal tubule cells

Abstract

Keywords

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