Enzymatic synthesis of 2′-deoxypseudouridylic acid

Thomas I. Kalman

doi:10.1016/S0006-291X(72)80101-3

Enzymatic synthesis of 2′-deoxypseudouridylic acid

Thomas I. Kalman

Chemistry

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8 Scopus citations

Abstract

A partially purified pseudouridylate synthetase preparation of Agrobacterium tumefaciens B6 was found to catalyze the reaction between uracil and 2-deoxyribose-5-phosphate. The product of this enzymic reaction was susceptible to cleavage by snake venom 5′-nucleotidase to yield a hitherto undescribed nucleoside, which resembled 8-pseudouridine in its ultraviolet spectral characteristics and its reactivity toward acrylonitrile. The initial synthetic product of the enzymic reaction was identified as 2′-deoxypseudouridylic acid.

Original language	English
Pages (from-to)	1194-1200
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	46
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(72)80101-3
State	Published - Feb 16 1972

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title = "Enzymatic synthesis of 2′-deoxypseudouridylic acid",

abstract = "A partially purified pseudouridylate synthetase preparation of Agrobacterium tumefaciens B6 was found to catalyze the reaction between uracil and 2-deoxyribose-5-phosphate. The product of this enzymic reaction was susceptible to cleavage by snake venom 5′-nucleotidase to yield a hitherto undescribed nucleoside, which resembled 8-pseudouridine in its ultraviolet spectral characteristics and its reactivity toward acrylonitrile. The initial synthetic product of the enzymic reaction was identified as 2′-deoxypseudouridylic acid.",

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N2 - A partially purified pseudouridylate synthetase preparation of Agrobacterium tumefaciens B6 was found to catalyze the reaction between uracil and 2-deoxyribose-5-phosphate. The product of this enzymic reaction was susceptible to cleavage by snake venom 5′-nucleotidase to yield a hitherto undescribed nucleoside, which resembled 8-pseudouridine in its ultraviolet spectral characteristics and its reactivity toward acrylonitrile. The initial synthetic product of the enzymic reaction was identified as 2′-deoxypseudouridylic acid.

AB - A partially purified pseudouridylate synthetase preparation of Agrobacterium tumefaciens B6 was found to catalyze the reaction between uracil and 2-deoxyribose-5-phosphate. The product of this enzymic reaction was susceptible to cleavage by snake venom 5′-nucleotidase to yield a hitherto undescribed nucleoside, which resembled 8-pseudouridine in its ultraviolet spectral characteristics and its reactivity toward acrylonitrile. The initial synthetic product of the enzymic reaction was identified as 2′-deoxypseudouridylic acid.

UR - https://www.scopus.com/pages/publications/0015512074

U2 - 10.1016/S0006-291X(72)80101-3

DO - 10.1016/S0006-291X(72)80101-3

M3 - Article

C2 - 5062424

AN - SCOPUS:0015512074

SN - 0006-291X

VL - 46

SP - 1194

EP - 1200

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Enzymatic synthesis of 2′-deoxypseudouridylic acid

Abstract

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