Enrichment of midsized RNAs with manganese chloride precipitation

Dillon Friday; Paul Freidhoff; Tilman Baumstark; Michael F. Bruist

doi:10.1016/j.ab.2018.09.003

Enrichment of midsized RNAs with manganese chloride precipitation

Dillon Friday
, Paul Freidhoff
, Tilman Baumstark
, Michael F. Bruist

Faculty Affairs Office

University of Sciences in Philadelphia
Campbell Soup Company

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Enrichment of specific RNAs is important for RNA analysis. MnCl₂ has been used previously to enrich viroid RNA fractions from total RNA from infected plants. We have expanded this method to show that MnCl₂ can enrich single-stranded as well as structured RNAs of 450 nt and below from a total RNA preparation. We have applied this method to map the transcription start sites of a PSTVd transcript from total RNA from yeast under conditions where the RNA was previously undetectable.

Original language	English
Pages (from-to)	56-59
Number of pages	4
Journal	Analytical Biochemistry
Volume	560
DOIs	https://doi.org/10.1016/j.ab.2018.09.003
State	Published - Nov 1 2018

Keywords

In vivo RNA processing
Polyethylene glycol precipitation
Primer extension
Viroid RNA

Access to Document

10.1016/j.ab.2018.09.003

Cite this

@article{ccf01888610440b1ac0a293be6cc289a,

title = "Enrichment of midsized RNAs with manganese chloride precipitation",

abstract = "Enrichment of specific RNAs is important for RNA analysis. MnCl2 has been used previously to enrich viroid RNA fractions from total RNA from infected plants. We have expanded this method to show that MnCl2 can enrich single-stranded as well as structured RNAs of 450 nt and below from a total RNA preparation. We have applied this method to map the transcription start sites of a PSTVd transcript from total RNA from yeast under conditions where the RNA was previously undetectable.",

keywords = "In vivo RNA processing, Polyethylene glycol precipitation, Primer extension, Viroid RNA",

author = "Dillon Friday and Paul Freidhoff and Tilman Baumstark and Bruist, \{Michael F.\}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

year = "2018",

month = nov,

day = "1",

doi = "10.1016/j.ab.2018.09.003",

language = "English",

volume = "560",

pages = "56--59",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Enrichment of midsized RNAs with manganese chloride precipitation

AU - Friday, Dillon

AU - Freidhoff, Paul

AU - Baumstark, Tilman

AU - Bruist, Michael F.

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Enrichment of specific RNAs is important for RNA analysis. MnCl2 has been used previously to enrich viroid RNA fractions from total RNA from infected plants. We have expanded this method to show that MnCl2 can enrich single-stranded as well as structured RNAs of 450 nt and below from a total RNA preparation. We have applied this method to map the transcription start sites of a PSTVd transcript from total RNA from yeast under conditions where the RNA was previously undetectable.

AB - Enrichment of specific RNAs is important for RNA analysis. MnCl2 has been used previously to enrich viroid RNA fractions from total RNA from infected plants. We have expanded this method to show that MnCl2 can enrich single-stranded as well as structured RNAs of 450 nt and below from a total RNA preparation. We have applied this method to map the transcription start sites of a PSTVd transcript from total RNA from yeast under conditions where the RNA was previously undetectable.

KW - In vivo RNA processing

KW - Polyethylene glycol precipitation

KW - Primer extension

KW - Viroid RNA

UR - https://www.scopus.com/pages/publications/85053377328

U2 - 10.1016/j.ab.2018.09.003

DO - 10.1016/j.ab.2018.09.003

M3 - Article

C2 - 30193930

AN - SCOPUS:85053377328

SN - 0003-2697

VL - 560

SP - 56

EP - 59

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Enrichment of midsized RNAs with manganese chloride precipitation

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this