End-Column Chemiluminescence Detector for Capillary Electrophoresis

Rajeev Dadoo; Anita G. Seto; Luis A. Colón; Richard N. Zare

doi:10.1021/ac00074a020

End-Column Chemiluminescence Detector for Capillary Electrophoresis

Rajeev Dadoo
, Anita G. Seto
, Luis A. Colón
, Richard N. Zare

Chemistry

Stanford University

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

A chemiluminescence detector is designed for capillary electrophoresis in which the signal is generated at the column outlet. As the analytes emerge from the column, they react with chemiluminescent reagents to produce visible light that is transported by a fiber optic to a photomultiplier tube. The luminol chemiluminescence and the firefly luciferase bioluminescence reactions are adapted for use with this type of detection scheme. The reactions yield concentration detection limits of 2 × 10⁻⁸ M for luminol and 5 × 10⁻⁹ M for ATP, which are approximately 3 orders of magnitude lower than those obtained with absorbance.

Original language	English
Pages (from-to)	303-306
Number of pages	4
Journal	Analytical Chemistry
Volume	66
Issue number	2
DOIs	https://doi.org/10.1021/ac00074a020
State	Published - Jan 15 1994

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title = "End-Column Chemiluminescence Detector for Capillary Electrophoresis",

abstract = "A chemiluminescence detector is designed for capillary electrophoresis in which the signal is generated at the column outlet. As the analytes emerge from the column, they react with chemiluminescent reagents to produce visible light that is transported by a fiber optic to a photomultiplier tube. The luminol chemiluminescence and the firefly luciferase bioluminescence reactions are adapted for use with this type of detection scheme. The reactions yield concentration detection limits of 2 × 10−8 M for luminol and 5 × 10−9 M for ATP, which are approximately 3 orders of magnitude lower than those obtained with absorbance.",

author = "Rajeev Dadoo and Seto, \{Anita G.\} and Col{\'o}n, \{Luis A.\} and Zare, \{Richard N.\}",

year = "1994",

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language = "English",

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T1 - End-Column Chemiluminescence Detector for Capillary Electrophoresis

AU - Dadoo, Rajeev

AU - Seto, Anita G.

AU - Colón, Luis A.

AU - Zare, Richard N.

PY - 1994/1/15

Y1 - 1994/1/15

N2 - A chemiluminescence detector is designed for capillary electrophoresis in which the signal is generated at the column outlet. As the analytes emerge from the column, they react with chemiluminescent reagents to produce visible light that is transported by a fiber optic to a photomultiplier tube. The luminol chemiluminescence and the firefly luciferase bioluminescence reactions are adapted for use with this type of detection scheme. The reactions yield concentration detection limits of 2 × 10−8 M for luminol and 5 × 10−9 M for ATP, which are approximately 3 orders of magnitude lower than those obtained with absorbance.

AB - A chemiluminescence detector is designed for capillary electrophoresis in which the signal is generated at the column outlet. As the analytes emerge from the column, they react with chemiluminescent reagents to produce visible light that is transported by a fiber optic to a photomultiplier tube. The luminol chemiluminescence and the firefly luciferase bioluminescence reactions are adapted for use with this type of detection scheme. The reactions yield concentration detection limits of 2 × 10−8 M for luminol and 5 × 10−9 M for ATP, which are approximately 3 orders of magnitude lower than those obtained with absorbance.

UR - https://www.scopus.com/pages/publications/0028226628

U2 - 10.1021/ac00074a020

DO - 10.1021/ac00074a020

M3 - Article

AN - SCOPUS:0028226628

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VL - 66

SP - 303

EP - 306

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 2

ER -

End-Column Chemiluminescence Detector for Capillary Electrophoresis

Abstract

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