Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography

Diandra Doppler; Mukul Sonker; Ana Egatz-Gomez; Garrett Nelson; Mohammad Towshif Rabbani; Jorvani Cruz Villarreal; Reza Nazari; Sahba Zaare; Darren Thifault; Sabine Botha; Thomas Grant; Petra Fromme; Richard Kirian; Alexandra Ros

Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography

Diandra Doppler
, Mukul Sonker
, Ana Egatz-Gomez
, Garrett Nelson
, Mohammad Towshif Rabbani
, Jorvani Cruz Villarreal
, Reza Nazari
, Sahba Zaare
, Darren Thifault
, Sabine Botha
, Thomas Grant
, Petra Fromme
, Richard Kirian
, Alexandra Ros

Department of Structural Biology

Arizona State University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) is an emerging and growing field in structural discovery; one of its principal achievements is the ability to resolve the structure of intricate membrane proteins at room temperature. Because all XFELs currently operational are pulsed, up to 99% of the sample delivered to the X-ray beam during its 'off-time' is wasted. To solve this major problem of large sample consumption, we report a revolutionary sample saving injection method which is compatible with all current XFELs.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	997-998
Number of pages	2
ISBN (Electronic)	9781733419031
State	Published - 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: Oct 10 2021 → Oct 14 2021

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 10/14/21

Keywords

3D-printing
microfluidics
sample conservation
serial femtosecond crystallography

Cite this

Doppler, D., Sonker, M., Egatz-Gomez, A., Nelson, G., Rabbani, M. T., Villarreal, J. C., Nazari, R., Zaare, S., Thifault, D., Botha, S., Grant, T., Fromme, P., Kirian, R., & Ros, A. (2021). Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 997-998). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Doppler, Diandra ; Sonker, Mukul ; Egatz-Gomez, Ana et al. / Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 997-998 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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title = "Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography",

abstract = "Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) is an emerging and growing field in structural discovery; one of its principal achievements is the ability to resolve the structure of intricate membrane proteins at room temperature. Because all XFELs currently operational are pulsed, up to 99\% of the sample delivered to the X-ray beam during its 'off-time' is wasted. To solve this major problem of large sample consumption, we report a revolutionary sample saving injection method which is compatible with all current XFELs.",

keywords = "3D-printing, microfluidics, sample conservation, serial femtosecond crystallography",

author = "Diandra Doppler and Mukul Sonker and Ana Egatz-Gomez and Garrett Nelson and Rabbani, \{Mohammad Towshif\} and Villarreal, \{Jorvani Cruz\} and Reza Nazari and Sahba Zaare and Darren Thifault and Sabine Botha and Thomas Grant and Petra Fromme and Richard Kirian and Alexandra Ros",

note = "Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

language = "English",

series = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "997--998",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

}

Doppler, D, Sonker, M, Egatz-Gomez, A, Nelson, G, Rabbani, MT, Villarreal, JC, Nazari, R, Zaare, S, Thifault, D, Botha, S, Grant, T, Fromme, P, Kirian, R & Ros, A 2021, Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 997-998, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21.

Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography. / Doppler, Diandra; Sonker, Mukul; Egatz-Gomez, Ana et al.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 997-998 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography

AU - Doppler, Diandra

AU - Sonker, Mukul

AU - Egatz-Gomez, Ana

AU - Nelson, Garrett

AU - Rabbani, Mohammad Towshif

AU - Villarreal, Jorvani Cruz

AU - Nazari, Reza

AU - Zaare, Sahba

AU - Thifault, Darren

AU - Botha, Sabine

AU - Grant, Thomas

AU - Fromme, Petra

AU - Kirian, Richard

AU - Ros, Alexandra

PY - 2021

Y1 - 2021

N2 - Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) is an emerging and growing field in structural discovery; one of its principal achievements is the ability to resolve the structure of intricate membrane proteins at room temperature. Because all XFELs currently operational are pulsed, up to 99% of the sample delivered to the X-ray beam during its 'off-time' is wasted. To solve this major problem of large sample consumption, we report a revolutionary sample saving injection method which is compatible with all current XFELs.

AB - Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) is an emerging and growing field in structural discovery; one of its principal achievements is the ability to resolve the structure of intricate membrane proteins at room temperature. Because all XFELs currently operational are pulsed, up to 99% of the sample delivered to the X-ray beam during its 'off-time' is wasted. To solve this major problem of large sample consumption, we report a revolutionary sample saving injection method which is compatible with all current XFELs.

KW - 3D-printing

KW - microfluidics

KW - sample conservation

KW - serial femtosecond crystallography

UR - https://www.scopus.com/pages/publications/85136980055

M3 - Conference contribution

AN - SCOPUS:85136980055

T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 997

EP - 998

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Doppler D, Sonker M, Egatz-Gomez A, Nelson G, Rabbani MT, Villarreal JC et al. Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 997-998. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Electronically Stimulated Segmented Flow for Reduced Sample Consumption During Serial Femtosecond Crystallography

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Publication series

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Keywords

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