TY - GEN
T1 - Effects of keratinocyte growth factor on in vitro engineered, genetically modified human epidermis
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
AU - Andreadis, Stelios T.
AU - Yarmush, Martin L.
AU - Morgan, Jeffrey R.
PY - 1999
Y1 - 1999
N2 - Keratinocyte growth factor (KGF) is a paracrine mediator of epithelial morphogenesis and reepithelialization following burn or injury. We used recombinant retroviruses to introduce the KGF gene in an in vitro engineered human epidermis and evaluated its potential as a wound-healing factor. In addition, we examined the effects of exogenously added KGF (paracrine) and KGF secreted by modified keratinocytes (autocrine). Microscopic evaluation of tissue sections showed KGF-expressing (modified) and KGF-treated (exogenous KGF) grafts with pronounced hyperthickening, elongation of the basal cells and flattening of the rete-ridges, as compared to controls. KGF induced a ripple-like pattern in the junction of stratum corneum and granular layers, similar to the rete-ridge pattern of unmodified grafts. Quantitative immunostaining revealed that KGF significantly increased proliferation of basal and suprabasal cells, similar to a psoriatic epidermis. In addition, KGF delayed the differentiation program of stratified epidermis, as suggested by the expression patterns of differentiation specific markers. All grafts developed an effective barrier as evaluated by measurements of surface hydration.
AB - Keratinocyte growth factor (KGF) is a paracrine mediator of epithelial morphogenesis and reepithelialization following burn or injury. We used recombinant retroviruses to introduce the KGF gene in an in vitro engineered human epidermis and evaluated its potential as a wound-healing factor. In addition, we examined the effects of exogenously added KGF (paracrine) and KGF secreted by modified keratinocytes (autocrine). Microscopic evaluation of tissue sections showed KGF-expressing (modified) and KGF-treated (exogenous KGF) grafts with pronounced hyperthickening, elongation of the basal cells and flattening of the rete-ridges, as compared to controls. KGF induced a ripple-like pattern in the junction of stratum corneum and granular layers, similar to the rete-ridge pattern of unmodified grafts. Quantitative immunostaining revealed that KGF significantly increased proliferation of basal and suprabasal cells, similar to a psoriatic epidermis. In addition, KGF delayed the differentiation program of stratified epidermis, as suggested by the expression patterns of differentiation specific markers. All grafts developed an effective barrier as evaluated by measurements of surface hydration.
UR - https://www.scopus.com/pages/publications/0033341076
M3 - Conference contribution
AN - SCOPUS:0033341076
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 132
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
Y2 - 13 October 1999 through 16 October 1999
ER -