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Effect of starvation and sampling time on plasma alkaline phosphatase activity and calcium homeostasis in the rat

  • C. S. Thompson
  • , D. P. Mikhailidis
  • , D. S. Gill
  • , J. Y. Jeremy
  • , J. L. Bell
  • , P. Dandona
  • Royal Free London NHS Foundation Trust
  • Metabolic Unit

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The effect of starvation and sampling time on plasma alkaline phosphatase activity, total plasma calcium concentration and whole blood ionized calcium concentration was determined in the rat. Starvation caused a significant fall in total and ionized calcium concentrations as well as in alkaline phosphatase activity. These changes were accompanied by a fall in whole blood pH and an increase in the anion gap and a decrease in urinary excretion of calcium. These indices were restored to normal following refeeding. There was no change in serum 25-OH vitamin D concentrations following starvation for 3 days. Alkaline phosphatase activity showed a pattern compatible with the presence of a circadian rhythm when sampling took place between 0800 and 1800 h. Total and ionized calcium concentrations did not show such a rhythm when animals were fed the present diet.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalLaboratory Animals
Volume23
Issue number1
DOIs
StatePublished - Jan 1 1989

Keywords

  • ALKALINE PHOSPHATASE
  • CALCIUM
  • STARVATION

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