Effect of androgens on adrenal cortical 11β-hydroxylation

Howard D. Colby; Alexander C. Brownie

doi:10.1016/0022-4731(71)90040-9

Effect of androgens on adrenal cortical 11β-hydroxylation

Howard D. Colby
, Alexander C. Brownie

Biochemistry

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The effects of the chronic administration of testosterone, androstenedione and DHA upon adrenal cortical function in the rat were compared. Only testosterone induced hypertensive disease and decreased 11β- and 18-hydroxylase activity in vitro. Significant decreases in adrenal mitochondrial cytochrome P-450 levels were apparent in all androgen-treated groups. Testosterone administration also resulted in a significant increment in the concentration of cytochromes a + a₃. The DOC-induced difference spectrum was reduced in all androgen-treated groups when expressed per mg of protein but only in mitochondria from testosterone-treated rats when calculated per nanomole of cytochrome P-450. The results suggest that the inhibition of the 11β- and 18-hydroxylation of DOC by testosterone is the result of competitive binding to cytochrome P-450 and not the induced decrease in cytochrome P-450 levels per se.

Original language	English
Pages (from-to)	237-244
Number of pages	8
Journal	Journal of Steroid Biochemistry
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/0022-4731(71)90040-9
State	Published - Sep 1971

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title = "Effect of androgens on adrenal cortical 11β-hydroxylation",

abstract = "The effects of the chronic administration of testosterone, androstenedione and DHA upon adrenal cortical function in the rat were compared. Only testosterone induced hypertensive disease and decreased 11β- and 18-hydroxylase activity in vitro. Significant decreases in adrenal mitochondrial cytochrome P-450 levels were apparent in all androgen-treated groups. Testosterone administration also resulted in a significant increment in the concentration of cytochromes a + a3. The DOC-induced difference spectrum was reduced in all androgen-treated groups when expressed per mg of protein but only in mitochondria from testosterone-treated rats when calculated per nanomole of cytochrome P-450. The results suggest that the inhibition of the 11β- and 18-hydroxylation of DOC by testosterone is the result of competitive binding to cytochrome P-450 and not the induced decrease in cytochrome P-450 levels per se.",

author = "Colby, \{Howard D.\} and Brownie, \{Alexander C.\}",

year = "1971",

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doi = "10.1016/0022-4731(71)90040-9",

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T1 - Effect of androgens on adrenal cortical 11β-hydroxylation

AU - Colby, Howard D.

AU - Brownie, Alexander C.

PY - 1971/9

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N2 - The effects of the chronic administration of testosterone, androstenedione and DHA upon adrenal cortical function in the rat were compared. Only testosterone induced hypertensive disease and decreased 11β- and 18-hydroxylase activity in vitro. Significant decreases in adrenal mitochondrial cytochrome P-450 levels were apparent in all androgen-treated groups. Testosterone administration also resulted in a significant increment in the concentration of cytochromes a + a3. The DOC-induced difference spectrum was reduced in all androgen-treated groups when expressed per mg of protein but only in mitochondria from testosterone-treated rats when calculated per nanomole of cytochrome P-450. The results suggest that the inhibition of the 11β- and 18-hydroxylation of DOC by testosterone is the result of competitive binding to cytochrome P-450 and not the induced decrease in cytochrome P-450 levels per se.

AB - The effects of the chronic administration of testosterone, androstenedione and DHA upon adrenal cortical function in the rat were compared. Only testosterone induced hypertensive disease and decreased 11β- and 18-hydroxylase activity in vitro. Significant decreases in adrenal mitochondrial cytochrome P-450 levels were apparent in all androgen-treated groups. Testosterone administration also resulted in a significant increment in the concentration of cytochromes a + a3. The DOC-induced difference spectrum was reduced in all androgen-treated groups when expressed per mg of protein but only in mitochondria from testosterone-treated rats when calculated per nanomole of cytochrome P-450. The results suggest that the inhibition of the 11β- and 18-hydroxylation of DOC by testosterone is the result of competitive binding to cytochrome P-450 and not the induced decrease in cytochrome P-450 levels per se.

UR - https://www.scopus.com/pages/publications/49649148504

U2 - 10.1016/0022-4731(71)90040-9

DO - 10.1016/0022-4731(71)90040-9

M3 - Article

AN - SCOPUS:49649148504

SN - 0022-4731

VL - 2

SP - 237

EP - 244

JO - Journal of Steroid Biochemistry

JF - Journal of Steroid Biochemistry

IS - 3

ER -

Effect of androgens on adrenal cortical 11β-hydroxylation

Abstract

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