Effect of acth on cholesterol side-chain cleavage in rat adrenal mitochondria

Alexander C. Brownie; Evan R. Simpson; Colin R. Jefcoate; George S. Boyd; William H. Orme-Johnson; Helmut Beinert

doi:10.1016/S0006-291X(72)80164-5

Effect of acth on cholesterol side-chain cleavage in rat adrenal mitochondria

Alexander C. Brownie
, Evan R. Simpson
, Colin R. Jefcoate
, George S. Boyd
, William H. Orme-Johnson
, Helmut Beinert

Biochemistry

University of Edinburgh
University of Wisconsin-Madison

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

The acute effect of ether stress on cholesterol side chain cleavage and cytochrome P-450 has been studied in intact rat adrenal mitochondria using light absorption and EPR spectroscopy. The effect of stress is to increase the ratio of high-spin to low-spin oxidized cytochrome P-450 and this is accompanied by an increase in the rate of pregnenolone synthesis from adrenal mitochondrial cholesterol. Cycloheximide pre-treatment prevented these effects of ether stress. The action of ACTH appears to involve an increase in the association of cholesterol with cholesterol side chain cleavage cytochrome P-450.

Original language	English
Pages (from-to)	483-490
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	46
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(72)80164-5
State	Published - Jan 31 1972

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title = "Effect of acth on cholesterol side-chain cleavage in rat adrenal mitochondria",

abstract = "The acute effect of ether stress on cholesterol side chain cleavage and cytochrome P-450 has been studied in intact rat adrenal mitochondria using light absorption and EPR spectroscopy. The effect of stress is to increase the ratio of high-spin to low-spin oxidized cytochrome P-450 and this is accompanied by an increase in the rate of pregnenolone synthesis from adrenal mitochondrial cholesterol. Cycloheximide pre-treatment prevented these effects of ether stress. The action of ACTH appears to involve an increase in the association of cholesterol with cholesterol side chain cleavage cytochrome P-450.",

author = "Brownie, \{Alexander C.\} and Simpson, \{Evan R.\} and Jefcoate, \{Colin R.\} and Boyd, \{George S.\} and Orme-Johnson, \{William H.\} and Helmut Beinert",

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T1 - Effect of acth on cholesterol side-chain cleavage in rat adrenal mitochondria

AU - Brownie, Alexander C.

AU - Simpson, Evan R.

AU - Jefcoate, Colin R.

AU - Boyd, George S.

AU - Orme-Johnson, William H.

AU - Beinert, Helmut

PY - 1972/1/31

Y1 - 1972/1/31

N2 - The acute effect of ether stress on cholesterol side chain cleavage and cytochrome P-450 has been studied in intact rat adrenal mitochondria using light absorption and EPR spectroscopy. The effect of stress is to increase the ratio of high-spin to low-spin oxidized cytochrome P-450 and this is accompanied by an increase in the rate of pregnenolone synthesis from adrenal mitochondrial cholesterol. Cycloheximide pre-treatment prevented these effects of ether stress. The action of ACTH appears to involve an increase in the association of cholesterol with cholesterol side chain cleavage cytochrome P-450.

AB - The acute effect of ether stress on cholesterol side chain cleavage and cytochrome P-450 has been studied in intact rat adrenal mitochondria using light absorption and EPR spectroscopy. The effect of stress is to increase the ratio of high-spin to low-spin oxidized cytochrome P-450 and this is accompanied by an increase in the rate of pregnenolone synthesis from adrenal mitochondrial cholesterol. Cycloheximide pre-treatment prevented these effects of ether stress. The action of ACTH appears to involve an increase in the association of cholesterol with cholesterol side chain cleavage cytochrome P-450.

UR - https://www.scopus.com/pages/publications/0015530515

U2 - 10.1016/S0006-291X(72)80164-5

DO - 10.1016/S0006-291X(72)80164-5

M3 - Article

C2 - 4333417

AN - SCOPUS:0015530515

SN - 0006-291X

VL - 46

SP - 483

EP - 490

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Effect of acth on cholesterol side-chain cleavage in rat adrenal mitochondria

Abstract

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