Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry

Tracey M. Lewandowski; Peng An; Carlo P. Ramil; Ming Fang; Qing Lin

doi:10.1039/d2cb00107a

Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry

Tracey M. Lewandowski
, Peng An
, Carlo P. Ramil
, Ming Fang
, Qing Lin

Chemistry

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells.

Original language	English
Pages (from-to)	702-706
Number of pages	5
Journal	RSC Chemical Biology
Volume	3
Issue number	6
DOIs	https://doi.org/10.1039/d2cb00107a
State	Published - May 17 2022

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10.1039/d2cb00107a

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title = "Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry",

abstract = "To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells.",

author = "Lewandowski, \{Tracey M.\} and Peng An and Ramil, \{Carlo P.\} and Ming Fang and Qing Lin",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s).",

year = "2022",

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doi = "10.1039/d2cb00107a",

language = "English",

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journal = "RSC Chemical Biology",

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TY - JOUR

T1 - Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry

AU - Lewandowski, Tracey M.

AU - An, Peng

AU - Ramil, Carlo P.

AU - Fang, Ming

AU - Lin, Qing

PY - 2022/5/17

Y1 - 2022/5/17

N2 - To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells.

AB - To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells.

UR - https://www.scopus.com/pages/publications/85133173258

U2 - 10.1039/d2cb00107a

DO - 10.1039/d2cb00107a

M3 - Article

AN - SCOPUS:85133173258

SN - 2633-0679

VL - 3

SP - 702

EP - 706

JO - RSC Chemical Biology

JF - RSC Chemical Biology

IS - 6

ER -

Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry

Abstract

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