D1-dopamine receptor activation mediates [3H]acetylcholine release from rabbit retina

J. G. Hensler; M. L. Dubocovich

doi:10.1016/0006-8993(86)91506-4

D₁-dopamine receptor activation mediates [³H]acetylcholine release from rabbit retina

J. G. Hensler
, M. L. Dubocovich

Pharmacology and Toxicology

Northwestern University

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Dopamine and dopamine receptor agonists (0.1 μM-10 mM) evoked, in a concentration-dependent manner, calcium-dependent release of [³H]acetylcholine from the rabbit retina labeled in vitro with [³H]choline. This stimulatory effect of dopamine was antagonized by SCH 23390 and by α-flupenthixol, but not by S-sulpiride or propranolol. Increases in intracellular cyclic AMP by forskolin, 3-isobutyl-1-methylxanthine and 8-bromo-cyclic AMP also evoked calcium-dependent release of [³H]acetylcholine. These results indicate that activation of a receptor with the biochemical and pharmacological characteristics of a D₁-dopamine receptor mediates calcium-dependent release of acetylcholine from rabbit retina, possibly through a cyclic AMP-dependent process.

Original language	English
Pages (from-to)	407-412
Number of pages	6
Journal	Brain Research
Volume	398
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(86)91506-4
State	Published - Nov 29 1986

Keywords

D-dopamine receptor
Rabbit retina
SCH 23390
SKF 38398
SKF 82526
[H]Acetylcholine release

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10.1016/0006-8993(86)91506-4

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title = "D1-dopamine receptor activation mediates [3H]acetylcholine release from rabbit retina",

abstract = "Dopamine and dopamine receptor agonists (0.1 μM-10 mM) evoked, in a concentration-dependent manner, calcium-dependent release of [3H]acetylcholine from the rabbit retina labeled in vitro with [3H]choline. This stimulatory effect of dopamine was antagonized by SCH 23390 and by α-flupenthixol, but not by S-sulpiride or propranolol. Increases in intracellular cyclic AMP by forskolin, 3-isobutyl-1-methylxanthine and 8-bromo-cyclic AMP also evoked calcium-dependent release of [3H]acetylcholine. These results indicate that activation of a receptor with the biochemical and pharmacological characteristics of a D1-dopamine receptor mediates calcium-dependent release of acetylcholine from rabbit retina, possibly through a cyclic AMP-dependent process.",

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T1 - D1-dopamine receptor activation mediates [3H]acetylcholine release from rabbit retina

AU - Hensler, J. G.

AU - Dubocovich, M. L.

PY - 1986/11/29

Y1 - 1986/11/29

N2 - Dopamine and dopamine receptor agonists (0.1 μM-10 mM) evoked, in a concentration-dependent manner, calcium-dependent release of [3H]acetylcholine from the rabbit retina labeled in vitro with [3H]choline. This stimulatory effect of dopamine was antagonized by SCH 23390 and by α-flupenthixol, but not by S-sulpiride or propranolol. Increases in intracellular cyclic AMP by forskolin, 3-isobutyl-1-methylxanthine and 8-bromo-cyclic AMP also evoked calcium-dependent release of [3H]acetylcholine. These results indicate that activation of a receptor with the biochemical and pharmacological characteristics of a D1-dopamine receptor mediates calcium-dependent release of acetylcholine from rabbit retina, possibly through a cyclic AMP-dependent process.

AB - Dopamine and dopamine receptor agonists (0.1 μM-10 mM) evoked, in a concentration-dependent manner, calcium-dependent release of [3H]acetylcholine from the rabbit retina labeled in vitro with [3H]choline. This stimulatory effect of dopamine was antagonized by SCH 23390 and by α-flupenthixol, but not by S-sulpiride or propranolol. Increases in intracellular cyclic AMP by forskolin, 3-isobutyl-1-methylxanthine and 8-bromo-cyclic AMP also evoked calcium-dependent release of [3H]acetylcholine. These results indicate that activation of a receptor with the biochemical and pharmacological characteristics of a D1-dopamine receptor mediates calcium-dependent release of acetylcholine from rabbit retina, possibly through a cyclic AMP-dependent process.

KW - D-dopamine receptor

KW - Rabbit retina

KW - SCH 23390

KW - SKF 38398

KW - SKF 82526

KW - [H]Acetylcholine release

UR - https://www.scopus.com/pages/publications/0022931158

U2 - 10.1016/0006-8993(86)91506-4

DO - 10.1016/0006-8993(86)91506-4

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AN - SCOPUS:0022931158

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SP - 407

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JO - Brain Research

JF - Brain Research

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ER -

D₁-dopamine receptor activation mediates [³H]acetylcholine release from rabbit retina

Abstract

Keywords

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