Distinguishing parallel from series circuits with a double knockdown procedure in human cell lines

Angela M. Gocher-Demske; Shuhang Dai; Arthur M. Edelman

doi:10.1016/j.xpro.2022.101890

Distinguishing parallel from series circuits with a double knockdown procedure in human cell lines

Angela M. Gocher-Demske
, Shuhang Dai
, Arthur M. Edelman

Pharmacology and Toxicology

University of Pittsburgh
SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Signaling cascades can act in series or in parallel. Here, we describe a convenient and robust protocol for dual, sequential knockdown of two proteins using RNA interference. We detail the steps for a quantitative mapping of signaling circuitry. We used this approach to study kinases in human ovarian cancer cells, but the protocol can be applied to many other posttranslational modifications. For complete details on the use and execution of this protocol, please refer to Gocher et al. (2017).¹

Original language	English
Article number	101890
Journal	STAR Protocols
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2022.101890
State	Published - Dec 16 2022

Keywords

Cell Biology
Molecular Biology
Protein Biochemistry
Signal Transduction

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10.1016/j.xpro.2022.101890

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abstract = "Signaling cascades can act in series or in parallel. Here, we describe a convenient and robust protocol for dual, sequential knockdown of two proteins using RNA interference. We detail the steps for a quantitative mapping of signaling circuitry. We used this approach to study kinases in human ovarian cancer cells, but the protocol can be applied to many other posttranslational modifications. For complete details on the use and execution of this protocol, please refer to Gocher et al. (2017).1",

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AU - Edelman, Arthur M.

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N2 - Signaling cascades can act in series or in parallel. Here, we describe a convenient and robust protocol for dual, sequential knockdown of two proteins using RNA interference. We detail the steps for a quantitative mapping of signaling circuitry. We used this approach to study kinases in human ovarian cancer cells, but the protocol can be applied to many other posttranslational modifications. For complete details on the use and execution of this protocol, please refer to Gocher et al. (2017).1

AB - Signaling cascades can act in series or in parallel. Here, we describe a convenient and robust protocol for dual, sequential knockdown of two proteins using RNA interference. We detail the steps for a quantitative mapping of signaling circuitry. We used this approach to study kinases in human ovarian cancer cells, but the protocol can be applied to many other posttranslational modifications. For complete details on the use and execution of this protocol, please refer to Gocher et al. (2017).1

KW - Cell Biology

KW - Molecular Biology

KW - Protein Biochemistry

KW - Signal Transduction

UR - https://www.scopus.com/pages/publications/85143508731

U2 - 10.1016/j.xpro.2022.101890

DO - 10.1016/j.xpro.2022.101890

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ER -

Distinguishing parallel from series circuits with a double knockdown procedure in human cell lines

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Keywords

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