Differentiation of strains of Actinobacillus actinomycetemcomitans by arbitrarily primed polymerase chain reaction

H. R. Preus; V. I. Haraszthy; J. J. Zambon; R. J. Genco

doi:10.1128/jcm.31.10.2773-2776.1993

Differentiation of strains of Actinobacillus actinomycetemcomitans by arbitrarily primed polymerase chain reaction

H. R. Preus
, V. I. Haraszthy
, J. J. Zambon
, R. J. Genco

SUNY Buffalo

Research output: Contribution to journal › Comment/debate

20 Scopus citations

Abstract

The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycler with a single synthetic primer (GGGTAACGCC) and reproducibly produced 14 different DNA amplification profiles (amplitypes). A. actinomycetemcomitans isolates from the same subject revealed the same amplitype. The arbitrarily primed polymerase chain reaction appears to be useful in characterizing human isolates of A. actinomycetemcomitans for studies of epidemiology and bacterial transmission.

Original language	English
Pages (from-to)	2773-2776
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	31
Issue number	10
DOIs	https://doi.org/10.1128/jcm.31.10.2773-2776.1993
State	Published - 1993

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10.1128/jcm.31.10.2773-2776.1993

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title = "Differentiation of strains of Actinobacillus actinomycetemcomitans by arbitrarily primed polymerase chain reaction",

abstract = "The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycler with a single synthetic primer (GGGTAACGCC) and reproducibly produced 14 different DNA amplification profiles (amplitypes). A. actinomycetemcomitans isolates from the same subject revealed the same amplitype. The arbitrarily primed polymerase chain reaction appears to be useful in characterizing human isolates of A. actinomycetemcomitans for studies of epidemiology and bacterial transmission.",

author = "Preus, \{H. R.\} and Haraszthy, \{V. I.\} and Zambon, \{J. J.\} and Genco, \{R. J.\}",

year = "1993",

doi = "10.1128/jcm.31.10.2773-2776.1993",

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pages = "2773--2776",

journal = "Journal of Clinical Microbiology",

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TY - JOUR

T1 - Differentiation of strains of Actinobacillus actinomycetemcomitans by arbitrarily primed polymerase chain reaction

AU - Preus, H. R.

AU - Haraszthy, V. I.

AU - Zambon, J. J.

AU - Genco, R. J.

PY - 1993

Y1 - 1993

N2 - The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycler with a single synthetic primer (GGGTAACGCC) and reproducibly produced 14 different DNA amplification profiles (amplitypes). A. actinomycetemcomitans isolates from the same subject revealed the same amplitype. The arbitrarily primed polymerase chain reaction appears to be useful in characterizing human isolates of A. actinomycetemcomitans for studies of epidemiology and bacterial transmission.

AB - The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycler with a single synthetic primer (GGGTAACGCC) and reproducibly produced 14 different DNA amplification profiles (amplitypes). A. actinomycetemcomitans isolates from the same subject revealed the same amplitype. The arbitrarily primed polymerase chain reaction appears to be useful in characterizing human isolates of A. actinomycetemcomitans for studies of epidemiology and bacterial transmission.

UR - https://www.scopus.com/pages/publications/0027484104

U2 - 10.1128/jcm.31.10.2773-2776.1993

DO - 10.1128/jcm.31.10.2773-2776.1993

M3 - Comment/debate

C2 - 8253980

AN - SCOPUS:0027484104

SN - 0095-1137

VL - 31

SP - 2773

EP - 2776

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 10

ER -

Differentiation of strains of Actinobacillus actinomycetemcomitans by arbitrarily primed polymerase chain reaction

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