Abstract
Aim: Determination of paritaprevir and ritonavir in rat liver tissue samples. Results: We successfully validated a UPLC-MS/MS method to measure paritaprevir and ritonavir in rat liver using deuterated internal standards (d8-paritapervir and d6-ritonavir). The method is linear from 20 to 20,000 and 5 to 10,000 pg on column for paritaprevir and ritonavir, respectively, and is normalized per milligram tissue. Interday and intraday variability ranged from 0.591 to 5.33% and accuracy ranged from -6.68 to 10.1% for quality control samples. The method was then applied to the measurement of paritaprevir and ritonavir in rat liver tissue samples from a pilot study. Conclusion: The validated method is suitable for measurement of paritaprevir and ritonavir within rat liver tissue samples for PK studies.
| Original language | English |
|---|---|
| Pages (from-to) | 1353-1363 |
| Number of pages | 11 |
| Journal | Bioanalysis |
| Volume | 8 |
| Issue number | 13 |
| DOIs | |
| State | Published - Jul 2016 |
Keywords
- UPLC-MS/MS
- core needle biopsy
- fine needle aspirate
- liver
- paritaprevir
- ritonavir
- validation
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