Determination of the antimicrobial growth promoter moenomycin-A in chicken litter

Sandra Pérez; Brenna E. McJury; Peter Eichhorn; Diana S. Aga

doi:10.1016/j.chroma.2007.10.053

Determination of the antimicrobial growth promoter moenomycin-A in chicken litter

Sandra Pérez
, Brenna E. McJury
, Peter Eichhorn
, Diana S. Aga

Chemistry

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

This study aimed to develop and optimize a method for the extraction and analysis of moenomycin antibiotics (a.k.a. flavomycin) in corn-based feed premix and in chicken litter. Moenomycin-A was isolated from chicken litter using pressurized liquid extraction followed by a solid-phase extraction (SPE) clean-up step. The highly lipophilic nature of moenomycin necessitated the use of the less hydrophobic sorbent, C₄-based SPE cartridge, and a higher temperature elution solvent, methanol at 50 °C, in order to obtain satisfactory percent recoveries. After clean-up, the sample was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). Various reversed-phase columns were examined, including C₁₈, CN, perfluorinated C₆, and a porous graphitic carbon. The set of conditions that gave the highest separation efficiency while still maintaining symmetrical peak shape was the C₁₈ column using the H₂O + 0.3% HCOOH and acetonitrile mobile phase.

Original language	English
Pages (from-to)	234-241
Number of pages	8
Journal	Journal of Chromatography A
Volume	1175
Issue number	2
DOIs	https://doi.org/10.1016/j.chroma.2007.10.053
State	Published - Dec 21 2007

Keywords

Chicken feed
Chicken litter
LC-ESI-MS
Moenomycin

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10.1016/j.chroma.2007.10.053

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title = "Determination of the antimicrobial growth promoter moenomycin-A in chicken litter",

abstract = "This study aimed to develop and optimize a method for the extraction and analysis of moenomycin antibiotics (a.k.a. flavomycin) in corn-based feed premix and in chicken litter. Moenomycin-A was isolated from chicken litter using pressurized liquid extraction followed by a solid-phase extraction (SPE) clean-up step. The highly lipophilic nature of moenomycin necessitated the use of the less hydrophobic sorbent, C4-based SPE cartridge, and a higher temperature elution solvent, methanol at 50 °C, in order to obtain satisfactory percent recoveries. After clean-up, the sample was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). Various reversed-phase columns were examined, including C18, CN, perfluorinated C6, and a porous graphitic carbon. The set of conditions that gave the highest separation efficiency while still maintaining symmetrical peak shape was the C18 column using the H2O + 0.3\% HCOOH and acetonitrile mobile phase.",

keywords = "Chicken feed, Chicken litter, LC-ESI-MS, Moenomycin",

author = "Sandra P{\'e}rez and McJury, \{Brenna E.\} and Peter Eichhorn and Aga, \{Diana S.\}",

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doi = "10.1016/j.chroma.2007.10.053",

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AU - Pérez, Sandra

AU - McJury, Brenna E.

AU - Eichhorn, Peter

AU - Aga, Diana S.

PY - 2007/12/21

Y1 - 2007/12/21

N2 - This study aimed to develop and optimize a method for the extraction and analysis of moenomycin antibiotics (a.k.a. flavomycin) in corn-based feed premix and in chicken litter. Moenomycin-A was isolated from chicken litter using pressurized liquid extraction followed by a solid-phase extraction (SPE) clean-up step. The highly lipophilic nature of moenomycin necessitated the use of the less hydrophobic sorbent, C4-based SPE cartridge, and a higher temperature elution solvent, methanol at 50 °C, in order to obtain satisfactory percent recoveries. After clean-up, the sample was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). Various reversed-phase columns were examined, including C18, CN, perfluorinated C6, and a porous graphitic carbon. The set of conditions that gave the highest separation efficiency while still maintaining symmetrical peak shape was the C18 column using the H2O + 0.3% HCOOH and acetonitrile mobile phase.

AB - This study aimed to develop and optimize a method for the extraction and analysis of moenomycin antibiotics (a.k.a. flavomycin) in corn-based feed premix and in chicken litter. Moenomycin-A was isolated from chicken litter using pressurized liquid extraction followed by a solid-phase extraction (SPE) clean-up step. The highly lipophilic nature of moenomycin necessitated the use of the less hydrophobic sorbent, C4-based SPE cartridge, and a higher temperature elution solvent, methanol at 50 °C, in order to obtain satisfactory percent recoveries. After clean-up, the sample was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). Various reversed-phase columns were examined, including C18, CN, perfluorinated C6, and a porous graphitic carbon. The set of conditions that gave the highest separation efficiency while still maintaining symmetrical peak shape was the C18 column using the H2O + 0.3% HCOOH and acetonitrile mobile phase.

KW - Chicken feed

KW - Chicken litter

KW - LC-ESI-MS

KW - Moenomycin

UR - https://www.scopus.com/pages/publications/36549019453

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DO - 10.1016/j.chroma.2007.10.053

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AN - SCOPUS:36549019453

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JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 2

ER -

Determination of the antimicrobial growth promoter moenomycin-A in chicken litter

Abstract

Keywords

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