Determination of protease inhibitors using liquid chromatography-tandem mass spectrometry

Valerie A. Frerichs; Robin DiFrancesco; Gene D. Morse

doi:10.1016/S1570-0232(02)01002-4

Determination of protease inhibitors using liquid chromatography-tandem mass spectrometry

Valerie A. Frerichs
, Robin DiFrancesco
, Gene D. Morse

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

A method for the analysis of six protease inhibitors and one metabolite has been developed and validated. Amprenavir, ritonavir, saquinavir, lopinavir, indinavir, nelfinavir, and an active metabolite of nelfinavir (M8) are quantitated using reversed-phase liquid chromatography coupled to tandem mass spectrometry, equipped with an electrospray ionization source (ESI-LC-MS-MS). The validation data presented here shows that the method allows the rugged analysis of these species from one aliquot. The evolution of complex drug interactions assessments and the clinical use of therapeutic drug monitoring for these antiretrovirals will be a potential immediate application of this method.

Original language	English
Pages (from-to)	393-403
Number of pages	11
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	787
Issue number	2
DOIs	https://doi.org/10.1016/S1570-0232(02)01002-4
State	Published - Apr 25 2003

Keywords

Protease inhibitors

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10.1016/S1570-0232(02)01002-4

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abstract = "A method for the analysis of six protease inhibitors and one metabolite has been developed and validated. Amprenavir, ritonavir, saquinavir, lopinavir, indinavir, nelfinavir, and an active metabolite of nelfinavir (M8) are quantitated using reversed-phase liquid chromatography coupled to tandem mass spectrometry, equipped with an electrospray ionization source (ESI-LC-MS-MS). The validation data presented here shows that the method allows the rugged analysis of these species from one aliquot. The evolution of complex drug interactions assessments and the clinical use of therapeutic drug monitoring for these antiretrovirals will be a potential immediate application of this method.",

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AU - DiFrancesco, Robin

AU - Morse, Gene D.

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N2 - A method for the analysis of six protease inhibitors and one metabolite has been developed and validated. Amprenavir, ritonavir, saquinavir, lopinavir, indinavir, nelfinavir, and an active metabolite of nelfinavir (M8) are quantitated using reversed-phase liquid chromatography coupled to tandem mass spectrometry, equipped with an electrospray ionization source (ESI-LC-MS-MS). The validation data presented here shows that the method allows the rugged analysis of these species from one aliquot. The evolution of complex drug interactions assessments and the clinical use of therapeutic drug monitoring for these antiretrovirals will be a potential immediate application of this method.

AB - A method for the analysis of six protease inhibitors and one metabolite has been developed and validated. Amprenavir, ritonavir, saquinavir, lopinavir, indinavir, nelfinavir, and an active metabolite of nelfinavir (M8) are quantitated using reversed-phase liquid chromatography coupled to tandem mass spectrometry, equipped with an electrospray ionization source (ESI-LC-MS-MS). The validation data presented here shows that the method allows the rugged analysis of these species from one aliquot. The evolution of complex drug interactions assessments and the clinical use of therapeutic drug monitoring for these antiretrovirals will be a potential immediate application of this method.

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JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

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ER -

Determination of protease inhibitors using liquid chromatography-tandem mass spectrometry

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Keywords

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