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Detection of Organophosphates Using an Acetyl Cholinesterase (AChE) Coated Microcantilever

  • Louisiana Tech University

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A microcantilever modified with a layer of acetyl cholinesterase (AChE) responding to paraoxon is reported in this work. Acetyl cholinesterase was immobilized on a microcantilever through a cross linker to a monolayer on gold surface of the microcantilever. The microcantilever underwent a maximum of 7 nm bending due to the inhibition of AChE by paraoxon that slightly changed the conformation of AChE.

Original languageEnglish
Pages (from-to)175-183
Number of pages9
JournalInstrumentation Science and Technology
Volume32
Issue number2
DOIs
StatePublished - Mar 2004

Keywords

  • Acetyl cholinesterase (AChE)
  • Adsorption-induced stress
  • Microcantilever
  • Organophosphate detection
  • Self-assembled monolayers (SAMs)

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