Detection of Cd(II) using antibody-modified microcantilever sensors

Sreepriya Velanki; Shantell Kelly; Thomas Thundat; Diane A. Blake; Hai Feng Ji

doi:10.1016/j.ultramic.2007.01.011

Detection of Cd(II) using antibody-modified microcantilever sensors

Sreepriya Velanki
, Shantell Kelly
, Thomas Thundat
, Diane A. Blake
, Hai Feng Ji

Department of Chemical and Biological Engineering

Louisiana Tech University
Tulane University

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

This work demonstrated the feasibility of detecting divalent Cd(II) ions using antibody-modified microcantilever (MCL) sensors. Different surface modification methods were compared and multilayer approach was found superior than others for MCL surface modifications for antibody-based Cd(II) sensor development. When the Cd(II)-EDTA complex sample is injected into the fluid cell where the MCL is held, the MCL bends upon the recognition of the Cd(II) complex by the antibody on the surface of the MCL. Control experiments showed that complex that does not contain Cd(II) did not cause any bending of the MCL. The detection limit of the sensor was approximately 10^-9 M. The reaction rate analysis indicated that Langmuir adsorption model is appropriate to describe the absorption of Cd(II)-EDTA-BSA on the antibody-covered MCL surface.

Original language	English
Pages (from-to)	1123-1128
Number of pages	6
Journal	Ultramicroscopy
Volume	107
Issue number	12
DOIs	https://doi.org/10.1016/j.ultramic.2007.01.011
State	Published - Nov 2007

Keywords

Adsorption-induced surface stress
Antibody
Cd-EDTA complex
MCL

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10.1016/j.ultramic.2007.01.011

Cite this

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title = "Detection of Cd(II) using antibody-modified microcantilever sensors",

abstract = "This work demonstrated the feasibility of detecting divalent Cd(II) ions using antibody-modified microcantilever (MCL) sensors. Different surface modification methods were compared and multilayer approach was found superior than others for MCL surface modifications for antibody-based Cd(II) sensor development. When the Cd(II)-EDTA complex sample is injected into the fluid cell where the MCL is held, the MCL bends upon the recognition of the Cd(II) complex by the antibody on the surface of the MCL. Control experiments showed that complex that does not contain Cd(II) did not cause any bending of the MCL. The detection limit of the sensor was approximately 10-9 M. The reaction rate analysis indicated that Langmuir adsorption model is appropriate to describe the absorption of Cd(II)-EDTA-BSA on the antibody-covered MCL surface.",

keywords = "Adsorption-induced surface stress, Antibody, Cd-EDTA complex, MCL",

author = "Sreepriya Velanki and Shantell Kelly and Thomas Thundat and Blake, \{Diane A.\} and Ji, \{Hai Feng\}",

year = "2007",

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doi = "10.1016/j.ultramic.2007.01.011",

language = "English",

volume = "107",

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T1 - Detection of Cd(II) using antibody-modified microcantilever sensors

AU - Velanki, Sreepriya

AU - Kelly, Shantell

AU - Thundat, Thomas

AU - Blake, Diane A.

AU - Ji, Hai Feng

PY - 2007/11

Y1 - 2007/11

N2 - This work demonstrated the feasibility of detecting divalent Cd(II) ions using antibody-modified microcantilever (MCL) sensors. Different surface modification methods were compared and multilayer approach was found superior than others for MCL surface modifications for antibody-based Cd(II) sensor development. When the Cd(II)-EDTA complex sample is injected into the fluid cell where the MCL is held, the MCL bends upon the recognition of the Cd(II) complex by the antibody on the surface of the MCL. Control experiments showed that complex that does not contain Cd(II) did not cause any bending of the MCL. The detection limit of the sensor was approximately 10-9 M. The reaction rate analysis indicated that Langmuir adsorption model is appropriate to describe the absorption of Cd(II)-EDTA-BSA on the antibody-covered MCL surface.

AB - This work demonstrated the feasibility of detecting divalent Cd(II) ions using antibody-modified microcantilever (MCL) sensors. Different surface modification methods were compared and multilayer approach was found superior than others for MCL surface modifications for antibody-based Cd(II) sensor development. When the Cd(II)-EDTA complex sample is injected into the fluid cell where the MCL is held, the MCL bends upon the recognition of the Cd(II) complex by the antibody on the surface of the MCL. Control experiments showed that complex that does not contain Cd(II) did not cause any bending of the MCL. The detection limit of the sensor was approximately 10-9 M. The reaction rate analysis indicated that Langmuir adsorption model is appropriate to describe the absorption of Cd(II)-EDTA-BSA on the antibody-covered MCL surface.

KW - Adsorption-induced surface stress

KW - Antibody

KW - Cd-EDTA complex

KW - MCL

UR - https://www.scopus.com/pages/publications/34548214306

U2 - 10.1016/j.ultramic.2007.01.011

DO - 10.1016/j.ultramic.2007.01.011

M3 - Article

C2 - 17353097

AN - SCOPUS:34548214306

SN - 0304-3991

VL - 107

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EP - 1128

JO - Ultramicroscopy

JF - Ultramicroscopy

IS - 12

ER -

Detection of Cd(II) using antibody-modified microcantilever sensors

Abstract

Keywords

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