TY - GEN
T1 - Detection of biological analytes using nanomechanical infrared spectroscopy with a nanoporous microcantilever
AU - Lee, Dongkyu
AU - Kim, Seonghwan
AU - Thundat, Thomas
PY - 2013
Y1 - 2013
N2 - The highly sensitive nanoporous cantilever beam without immobilized receptors was combined with highly selective mid-infrared (IR) spectroscopy for molecular recognition of analytes using characteristic molecular vibrations. Unlike conventional IR spectroscopy, in addition, the detection sensitivity and resolution are drastically enhanced by combining high power tunable quantum cascade laser with a nanoporous cantilever having large surface area, low modulus, and nanowell structures. Further, analytes can be easily loaded on the porous microcantilever without receptor due to nanowells. In addition, orthogonal signals, variations in the mass and IR spectrum, provide more reliable and quantitative results including physical as well as chemical information of samples. We have used this technique to rapidly identify single and double stranded DNA.
AB - The highly sensitive nanoporous cantilever beam without immobilized receptors was combined with highly selective mid-infrared (IR) spectroscopy for molecular recognition of analytes using characteristic molecular vibrations. Unlike conventional IR spectroscopy, in addition, the detection sensitivity and resolution are drastically enhanced by combining high power tunable quantum cascade laser with a nanoporous cantilever having large surface area, low modulus, and nanowell structures. Further, analytes can be easily loaded on the porous microcantilever without receptor due to nanowells. In addition, orthogonal signals, variations in the mass and IR spectrum, provide more reliable and quantitative results including physical as well as chemical information of samples. We have used this technique to rapidly identify single and double stranded DNA.
KW - nanoporous microcantilever
KW - Photothermal cantilever deflection spectroscopy
KW - quantum cascade laser
UR - https://www.scopus.com/pages/publications/84883763169
U2 - 10.1117/12.2017771
DO - 10.1117/12.2017771
M3 - Conference contribution
AN - SCOPUS:84883763169
SN - 9780819493040
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Nano-Bio Sensing, Imaging, and Spectroscopy
T2 - SPIE Nano-Bio Sensing, Imaging, and Spectroscopy
Y2 - 20 February 2013 through 23 February 2013
ER -