“Curing of common plasmids in gram-negative bacteria using a Cas9-based conjugative vector”

Kelly K. Yen; Austin J. Terlecky; Mingju Hao; Vanessa Cienfuegos; Albert Rojtman; Liang Chen; Barry N. Kreiswirth

doi:10.1016/j.mimet.2024.107047

“Curing of common plasmids in gram-negative bacteria using a Cas9-based conjugative vector”

Kelly K. Yen
, Austin J. Terlecky
, Mingju Hao
, Vanessa Cienfuegos
, Albert Rojtman
, Liang Chen
, Barry N. Kreiswirth

Pharmacy

Center for Discovery and Innovation
Shandong First Medical University & Shandong Academy of Medical Sciences
Universidad de Antioquia
Jersey Shore University Medical Center

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We report the creation of 17 Escherichia coli strains harboring the conjugative plasmid pLCasCureT with a CRISPR-Cas9 system to surgically “cure” the most common plasmids among Enterobacterales species. This approach can create isogenic pairs of strains to study host-plasmid interactions, correlate plasmid genotype and phenotype, and create plasmid-free cloning strains.

Original language	English
Article number	107047
Journal	Journal of Microbiological Methods
Volume	226
DOIs	https://doi.org/10.1016/j.mimet.2024.107047
State	Published - Nov 2024

Keywords

Antibiotic resistance
CRISPR-Cas9
Gram-negatives
Plasmid curing

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10.1016/j.mimet.2024.107047

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title = "“Curing of common plasmids in gram-negative bacteria using a Cas9-based conjugative vector”",

abstract = "We report the creation of 17 Escherichia coli strains harboring the conjugative plasmid pLCasCureT with a CRISPR-Cas9 system to surgically “cure” the most common plasmids among Enterobacterales species. This approach can create isogenic pairs of strains to study host-plasmid interactions, correlate plasmid genotype and phenotype, and create plasmid-free cloning strains.",

keywords = "Antibiotic resistance, CRISPR-Cas9, Gram-negatives, Plasmid curing",

author = "Yen, \{Kelly K.\} and Terlecky, \{Austin J.\} and Mingju Hao and Vanessa Cienfuegos and Albert Rojtman and Liang Chen and Kreiswirth, \{Barry N.\}",

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doi = "10.1016/j.mimet.2024.107047",

language = "English",

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T1 - “Curing of common plasmids in gram-negative bacteria using a Cas9-based conjugative vector”

AU - Yen, Kelly K.

AU - Terlecky, Austin J.

AU - Hao, Mingju

AU - Cienfuegos, Vanessa

AU - Rojtman, Albert

AU - Chen, Liang

AU - Kreiswirth, Barry N.

PY - 2024/11

Y1 - 2024/11

N2 - We report the creation of 17 Escherichia coli strains harboring the conjugative plasmid pLCasCureT with a CRISPR-Cas9 system to surgically “cure” the most common plasmids among Enterobacterales species. This approach can create isogenic pairs of strains to study host-plasmid interactions, correlate plasmid genotype and phenotype, and create plasmid-free cloning strains.

AB - We report the creation of 17 Escherichia coli strains harboring the conjugative plasmid pLCasCureT with a CRISPR-Cas9 system to surgically “cure” the most common plasmids among Enterobacterales species. This approach can create isogenic pairs of strains to study host-plasmid interactions, correlate plasmid genotype and phenotype, and create plasmid-free cloning strains.

KW - Antibiotic resistance

KW - CRISPR-Cas9

KW - Gram-negatives

KW - Plasmid curing

UR - https://www.scopus.com/pages/publications/85204498744

U2 - 10.1016/j.mimet.2024.107047

DO - 10.1016/j.mimet.2024.107047

M3 - Article

C2 - 39303991

AN - SCOPUS:85204498744

SN - 0167-7012

VL - 226

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

M1 - 107047

ER -

“Curing of common plasmids in gram-negative bacteria using a Cas9-based conjugative vector”

Abstract

Keywords

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