Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

Faithful Makita-Chingombe; Anthony T. Podany; Timothy Mykris; Farai Muzambi; Richard W. Browne; Andrew J. Ocque; Robin DiFrancesco; Lee C. Winchester; Courtney V. Fletcher; Tinashe Mudzviti; Charles C. Maponga; Gene D. Morse

doi:10.4102/AJLM.V10I1.1264

Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

Faithful Makita-Chingombe
, Anthony T. Podany
, Timothy Mykris
, Farai Muzambi
, Richard W. Browne
, Andrew J. Ocque
, Robin DiFrancesco
, Lee C. Winchester
, Courtney V. Fletcher
, Tinashe Mudzviti
, Charles C. Maponga
, Gene D. Morse

University of Zimbabwe
University of Nebraska Medical Center
SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

Abstract

An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20% difference.

Original language	English
Article number	a1264
Journal	African Journal of Laboratory Medicine
Volume	10
Issue number	1
DOIs	https://doi.org/10.4102/AJLM.V10I1.1264
State	Published - 2021

Keywords

Cross-validation
HIV
High-performance liquid chromatography
Root-cause analysis

Access to Document

10.4102/AJLM.V10I1.1264

Cite this

Makita-Chingombe, F., Podany, A. T., Mykris, T., Muzambi, F., Browne, R. W., Ocque, A. J., DiFrancesco, R., Winchester, L. C., Fletcher, C. V., Mudzviti, T., Maponga, C. C., & Morse, G. D. (2021). Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe. African Journal of Laboratory Medicine, 10(1), Article a1264. https://doi.org/10.4102/AJLM.V10I1.1264

@article{90d3306d07df402e8adbe05824f63ba2,

title = "Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe",

abstract = "An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20\% difference.",

keywords = "Cross-validation, HIV, High-performance liquid chromatography, Root-cause analysis",

author = "Faithful Makita-Chingombe and Podany, \{Anthony T.\} and Timothy Mykris and Farai Muzambi and Browne, \{Richard W.\} and Ocque, \{Andrew J.\} and Robin DiFrancesco and Winchester, \{Lee C.\} and Fletcher, \{Courtney V.\} and Tinashe Mudzviti and Maponga, \{Charles C.\} and Morse, \{Gene D.\}",

note = "Publisher Copyright: {\textcopyright} 2021. The Authors.",

year = "2021",

doi = "10.4102/AJLM.V10I1.1264",

language = "English",

volume = "10",

journal = "African Journal of Laboratory Medicine",

issn = "2225-2002",

publisher = "AOSIS (Pty) Ltd",

number = "1",

}

Makita-Chingombe, F, Podany, AT, Mykris, T, Muzambi, F, Browne, RW, Ocque, AJ, DiFrancesco, R, Winchester, LC, Fletcher, CV, Mudzviti, T, Maponga, CC & Morse, GD 2021, 'Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe', African Journal of Laboratory Medicine, vol. 10, no. 1, a1264. https://doi.org/10.4102/AJLM.V10I1.1264

TY - JOUR

T1 - Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

AU - Makita-Chingombe, Faithful

AU - Podany, Anthony T.

AU - Mykris, Timothy

AU - Muzambi, Farai

AU - Browne, Richard W.

AU - Ocque, Andrew J.

AU - DiFrancesco, Robin

AU - Winchester, Lee C.

AU - Fletcher, Courtney V.

AU - Mudzviti, Tinashe

AU - Maponga, Charles C.

AU - Morse, Gene D.

PY - 2021

Y1 - 2021

N2 - An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20% difference.

AB - An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20% difference.

KW - Cross-validation

KW - HIV

KW - High-performance liquid chromatography

KW - Root-cause analysis

UR - https://www.scopus.com/pages/publications/85111241579

U2 - 10.4102/AJLM.V10I1.1264

DO - 10.4102/AJLM.V10I1.1264

M3 - Article

AN - SCOPUS:85111241579

SN - 2225-2002

VL - 10

JO - African Journal of Laboratory Medicine

JF - African Journal of Laboratory Medicine

IS - 1

M1 - a1264

ER -

Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this