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CoPoP liposomes displaying stabilized clade C HIV-1 Env elicit tier 2 multiclade neutralization in rabbits

  • Annemart Koornneef
  • , Kanika Vanshylla
  • , Gijs Hardenberg
  • , Lucy Rutten
  • , Nika M. Strokappe
  • , Jeroen Tolboom
  • , Jessica Vreugdenhil
  • , Karin Feddes de Boer
  • , Aditya Perkasa
  • , Sven Blokland
  • , Judith A. Burger
  • , Wei Chiao Huang
  • , Jonathan F. Lovell
  • , Danielle van Manen
  • , Rogier W. Sanders
  • , Roland C. Zahn
  • , Hanneke Schuitemaker
  • , Johannes P.M. Langedijk
  • , Frank Wegmann
  • Johnson & Johnson
  • University of Amsterdam
  • SUNY Buffalo
  • ForgeBio

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

One of the strategies towards an effective HIV-1 vaccine is to elicit broadly neutralizing antibody responses that target the high HIV-1 Env diversity. Here, we present an HIV-1 vaccine candidate that consists of cobalt porphyrin-phospholipid (CoPoP) liposomes decorated with repaired and stabilized clade C HIV-1 Env trimers in a prefusion conformation. These particles exhibit high HIV-1 Env trimer decoration, serum stability and bind broadly neutralizing antibodies. Three sequential immunizations of female rabbits with CoPoP liposomes displaying a different clade C HIV-1 gp140 trimer at each dosing generate high HIV-1 Env-specific antibody responses. Additionally, serum neutralization is detectable against 18 of 20 multiclade tier 2 HIV-1 strains. Furthermore, the peak antibody titers induced by CoPoP liposomes can be recalled by subsequent heterologous immunization with Ad26-encoded membrane-bound stabilized Env antigens. Hence, a CoPoP liposome-based HIV-1 vaccine that can generate cross-clade neutralizing antibody immunity could potentially be a component of an efficacious HIV-1 vaccine.

Original languageEnglish
Article number3128
JournalNature Communications
Volume15
Issue number1
DOIs
StatePublished - Dec 2024

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