Cochlear mitochondrial DNA3867bp deletion in aged mice

Objectives. To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice. Methods. We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing. Results. mtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103 - nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17 - 19 month old mice (7/8) were significantly higher than 7 - 10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17 - 19 month old mice was higher than in 7 - 10 month old mice (P < 0. 001). Conclusion. Cochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis.