Chemical mapping of co-existing RNA structures

Astrid R.W. Schröder; Tilman Baumstark; Detlev Riesner

doi:10.1093/nar/26.14.3449

Chemical mapping of co-existing RNA structures

Astrid R.W. Schröder
, Tilman Baumstark
, Detlev Riesner

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Heinrich Heine University Düsseldorf

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15 Scopus citations

Abstract

In many cases RNA can assume co-existing or metastable structures preventing structure determination by chemical mapping. A novel method is described, by which RNA is modified with dimethyl sulphate without shifting the distribution of different structures. The different structures are then separated in native gel electrophoresis, and structure determination by primer extension can be carried out separately for each structure.

Original language	English
Pages (from-to)	3449-3450
Number of pages	2
Journal	Nucleic Acids Research
Volume	26
Issue number	14
DOIs	https://doi.org/10.1093/nar/26.14.3449
State	Published - Jul 15 1998

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title = "Chemical mapping of co-existing RNA structures",

abstract = "In many cases RNA can assume co-existing or metastable structures preventing structure determination by chemical mapping. A novel method is described, by which RNA is modified with dimethyl sulphate without shifting the distribution of different structures. The different structures are then separated in native gel electrophoresis, and structure determination by primer extension can be carried out separately for each structure.",

author = "Schr{\"o}der, \{Astrid R.W.\} and Tilman Baumstark and Detlev Riesner",

year = "1998",

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T1 - Chemical mapping of co-existing RNA structures

AU - Schröder, Astrid R.W.

AU - Baumstark, Tilman

AU - Riesner, Detlev

PY - 1998/7/15

Y1 - 1998/7/15

N2 - In many cases RNA can assume co-existing or metastable structures preventing structure determination by chemical mapping. A novel method is described, by which RNA is modified with dimethyl sulphate without shifting the distribution of different structures. The different structures are then separated in native gel electrophoresis, and structure determination by primer extension can be carried out separately for each structure.

AB - In many cases RNA can assume co-existing or metastable structures preventing structure determination by chemical mapping. A novel method is described, by which RNA is modified with dimethyl sulphate without shifting the distribution of different structures. The different structures are then separated in native gel electrophoresis, and structure determination by primer extension can be carried out separately for each structure.

UR - https://www.scopus.com/pages/publications/2642650356

U2 - 10.1093/nar/26.14.3449

DO - 10.1093/nar/26.14.3449

M3 - Article

C2 - 9649634

AN - SCOPUS:2642650356

SN - 0305-1048

VL - 26

SP - 3449

EP - 3450

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 14

ER -

Chemical mapping of co-existing RNA structures

Abstract

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