Characterization of polyclonal antibodies raised against a linear peptide determinant of desmoglein-3

Giovanni Angelini; Domenico Bonamonte; Mong Shang Lin; Alberta Lucchese; Abraham Mittelman; Rosario Serpico; Simone Simone; Animesh A. Sinha; Darja Kanduc

Characterization of polyclonal antibodies raised against a linear peptide determinant of desmoglein-3

Giovanni Angelini
, Domenico Bonamonte
, Mong Shang Lin
, Alberta Lucchese
, Abraham Mittelman
, Rosario Serpico
, Simone Simone
, Animesh A. Sinha
, Darja Kanduc

Department of Dermatology

University of Bari
Medical College of Wisconsin
New York Medical College

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Our labs are pursueing the goal of exactly defining the qualities of peptide antigenicity, immunogenicity and pathogenicity in order to develop safe specific immunotherapies by utilizing specific portions of disease-associated- proteins (DAPs). Using as a model the Pemphigus vulgaris antigen (PVA) desmoglein 3 (Dsg3), we have studied the murine humoral response against the Dsg3 amino acid 49-60 peptide sequence, previously characterized as sequence having low similarity to the mouse proteome. The results show that the low-similarity Dsg3_49-60REWVKFAKPCRE peptide does not elicit pathogenic antibodies.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of Experimental Therapeutics and Oncology
Volume	5
Issue number	1
State	Published - 2005

Keywords

Desmoglein 3
Low-similarity sequence
Pemphigus vulgaris
Peptide Immunotherapy
Proteomics

Cite this

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abstract = "Our labs are pursueing the goal of exactly defining the qualities of peptide antigenicity, immunogenicity and pathogenicity in order to develop safe specific immunotherapies by utilizing specific portions of disease-associated- proteins (DAPs). Using as a model the Pemphigus vulgaris antigen (PVA) desmoglein 3 (Dsg3), we have studied the murine humoral response against the Dsg3 amino acid 49-60 peptide sequence, previously characterized as sequence having low similarity to the mouse proteome. The results show that the low-similarity Dsg349-60REWVKFAKPCRE peptide does not elicit pathogenic antibodies.",

keywords = "Desmoglein 3, Low-similarity sequence, Pemphigus vulgaris, Peptide Immunotherapy, Proteomics",

author = "Giovanni Angelini and Domenico Bonamonte and Lin, \{Mong Shang\} and Alberta Lucchese and Abraham Mittelman and Rosario Serpico and Simone Simone and Sinha, \{Animesh A.\} and Darja Kanduc",

year = "2005",

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T1 - Characterization of polyclonal antibodies raised against a linear peptide determinant of desmoglein-3

AU - Angelini, Giovanni

AU - Bonamonte, Domenico

AU - Lin, Mong Shang

AU - Lucchese, Alberta

AU - Mittelman, Abraham

AU - Serpico, Rosario

AU - Simone, Simone

AU - Sinha, Animesh A.

AU - Kanduc, Darja

PY - 2005

Y1 - 2005

N2 - Our labs are pursueing the goal of exactly defining the qualities of peptide antigenicity, immunogenicity and pathogenicity in order to develop safe specific immunotherapies by utilizing specific portions of disease-associated- proteins (DAPs). Using as a model the Pemphigus vulgaris antigen (PVA) desmoglein 3 (Dsg3), we have studied the murine humoral response against the Dsg3 amino acid 49-60 peptide sequence, previously characterized as sequence having low similarity to the mouse proteome. The results show that the low-similarity Dsg349-60REWVKFAKPCRE peptide does not elicit pathogenic antibodies.

AB - Our labs are pursueing the goal of exactly defining the qualities of peptide antigenicity, immunogenicity and pathogenicity in order to develop safe specific immunotherapies by utilizing specific portions of disease-associated- proteins (DAPs). Using as a model the Pemphigus vulgaris antigen (PVA) desmoglein 3 (Dsg3), we have studied the murine humoral response against the Dsg3 amino acid 49-60 peptide sequence, previously characterized as sequence having low similarity to the mouse proteome. The results show that the low-similarity Dsg349-60REWVKFAKPCRE peptide does not elicit pathogenic antibodies.

KW - Desmoglein 3

KW - Low-similarity sequence

KW - Pemphigus vulgaris

KW - Peptide Immunotherapy

KW - Proteomics

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SN - 1359-4117

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JO - Journal of Experimental Therapeutics and Oncology

JF - Journal of Experimental Therapeutics and Oncology

IS - 1

ER -

Characterization of polyclonal antibodies raised against a linear peptide determinant of desmoglein-3

Abstract

Keywords

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