Characterization of a pentasaccharide in salivary mucin from the stumptail monkey, Macaca arctowes

L. A. Tabak; M. S. Reddy; M. J. Levine

doi:10.1016/0003-9969(82)90158-3

Characterization of a pentasaccharide in salivary mucin from the stumptail monkey, Macaca arctowes

L. A. Tabak
, M. S. Reddy
, M. J. Levine

Department of Oral Biology

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Oligosaccharides were prepared by alkaline cleavage and sodium borotritide reduction. Following gel filtration on Sephadex G-25, [³H]-oligosaccharides were further fractionated by anion-exchange and preparative paper chromatography. The principal neutral oligosaccharide contained GalNAcol:GlcNAc:Gal:Fuc (1:1:2:1). Using a combination of exoglycosidase digestion, periodate oxidation and methylation analysis by gas chromatography-mass spectrometry, it structure was determined to be: Gal β1→ 4 GlcNAc β1 → 3(Fuc α1 → 2 Gal 1 → 6)GalNAcol.

Original language	English
Pages (from-to)	297-303
Number of pages	7
Journal	Archives of Oral Biology
Volume	27
Issue number	4
DOIs	https://doi.org/10.1016/0003-9969(82)90158-3
State	Published - 1982

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title = "Characterization of a pentasaccharide in salivary mucin from the stumptail monkey, Macaca arctowes",

abstract = "Oligosaccharides were prepared by alkaline cleavage and sodium borotritide reduction. Following gel filtration on Sephadex G-25, [3H]-oligosaccharides were further fractionated by anion-exchange and preparative paper chromatography. The principal neutral oligosaccharide contained GalNAcol:GlcNAc:Gal:Fuc (1:1:2:1). Using a combination of exoglycosidase digestion, periodate oxidation and methylation analysis by gas chromatography-mass spectrometry, it structure was determined to be: Gal β1→ 4 GlcNAc β1 → 3(Fuc α1 → 2 Gal 1 → 6)GalNAcol.",

author = "Tabak, \{L. A.\} and Reddy, \{M. S.\} and Levine, \{M. J.\}",

year = "1982",

doi = "10.1016/0003-9969(82)90158-3",

language = "English",

volume = "27",

pages = "297--303",

journal = "Archives of Oral Biology",

issn = "0003-9969",

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T1 - Characterization of a pentasaccharide in salivary mucin from the stumptail monkey, Macaca arctowes

AU - Tabak, L. A.

AU - Reddy, M. S.

AU - Levine, M. J.

PY - 1982

Y1 - 1982

N2 - Oligosaccharides were prepared by alkaline cleavage and sodium borotritide reduction. Following gel filtration on Sephadex G-25, [3H]-oligosaccharides were further fractionated by anion-exchange and preparative paper chromatography. The principal neutral oligosaccharide contained GalNAcol:GlcNAc:Gal:Fuc (1:1:2:1). Using a combination of exoglycosidase digestion, periodate oxidation and methylation analysis by gas chromatography-mass spectrometry, it structure was determined to be: Gal β1→ 4 GlcNAc β1 → 3(Fuc α1 → 2 Gal 1 → 6)GalNAcol.

AB - Oligosaccharides were prepared by alkaline cleavage and sodium borotritide reduction. Following gel filtration on Sephadex G-25, [3H]-oligosaccharides were further fractionated by anion-exchange and preparative paper chromatography. The principal neutral oligosaccharide contained GalNAcol:GlcNAc:Gal:Fuc (1:1:2:1). Using a combination of exoglycosidase digestion, periodate oxidation and methylation analysis by gas chromatography-mass spectrometry, it structure was determined to be: Gal β1→ 4 GlcNAc β1 → 3(Fuc α1 → 2 Gal 1 → 6)GalNAcol.

UR - https://www.scopus.com/pages/publications/0020016146

U2 - 10.1016/0003-9969(82)90158-3

DO - 10.1016/0003-9969(82)90158-3

M3 - Article

C2 - 6953947

AN - SCOPUS:0020016146

SN - 0003-9969

VL - 27

SP - 297

EP - 303

JO - Archives of Oral Biology

JF - Archives of Oral Biology

IS - 4

ER -

Characterization of a pentasaccharide in salivary mucin from the stumptail monkey, Macaca arctowes

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