CCD video observation of microgravity crystallization of lysozyme and correlation with accelerometer data

E. H. Snell; T. J. Boggon; J. R. Helliwell; M. E. Moskowitz; A. Nadarajah

doi:10.1107/S0907444997007968

CCD video observation of microgravity crystallization of lysozyme and correlation with accelerometer data

E. H. Snell
, T. J. Boggon
, J. R. Helliwell
, M. E. Moskowitz
, A. Nadarajah

Department of Materials Design and Innovation

University of Manchester
NASA Glenn Research Center
University of Toledo

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Lysozyme has been crystallized using the ESA Advanced Protein Crystallization Facility onboard the NASA Space Shuttle Orbiter during the IML-2 mission. CCD video monitoring was used to follow the crystallization process and evaluate the growth rate. During the mission some tetragonal crystals were observed moving over distances of up to 200 μm. This was correlated with microgravity disturbances caused by firings of vernier jets on the Orbiter. Growth-rate measurement of a stationary crystal (which had nucleated on the growth reactor wall) showed spurts and lulls correlated with an onboard activity: astronaut exercise. The stepped growth rates may be responsible for the residual mosaic block structure seen in crystal mosaicity and topography measurements.

Original language	English
Pages (from-to)	747-755
Number of pages	9
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	53
Issue number	6
DOIs	https://doi.org/10.1107/S0907444997007968
State	Published - 1997

Access to Document

10.1107/S0907444997007968

Cite this

@article{02f9f1691890459bae4d786d331bcb36,

title = "CCD video observation of microgravity crystallization of lysozyme and correlation with accelerometer data",

abstract = "Lysozyme has been crystallized using the ESA Advanced Protein Crystallization Facility onboard the NASA Space Shuttle Orbiter during the IML-2 mission. CCD video monitoring was used to follow the crystallization process and evaluate the growth rate. During the mission some tetragonal crystals were observed moving over distances of up to 200 μm. This was correlated with microgravity disturbances caused by firings of vernier jets on the Orbiter. Growth-rate measurement of a stationary crystal (which had nucleated on the growth reactor wall) showed spurts and lulls correlated with an onboard activity: astronaut exercise. The stepped growth rates may be responsible for the residual mosaic block structure seen in crystal mosaicity and topography measurements.",

author = "Snell, \{E. H.\} and Boggon, \{T. J.\} and Helliwell, \{J. R.\} and Moskowitz, \{M. E.\} and A. Nadarajah",

year = "1997",

doi = "10.1107/S0907444997007968",

language = "English",

volume = "53",

pages = "747--755",

journal = "Acta Crystallographica Section D: Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

number = "6",

}

TY - JOUR

T1 - CCD video observation of microgravity crystallization of lysozyme and correlation with accelerometer data

AU - Snell, E. H.

AU - Boggon, T. J.

AU - Helliwell, J. R.

AU - Moskowitz, M. E.

AU - Nadarajah, A.

PY - 1997

Y1 - 1997

N2 - Lysozyme has been crystallized using the ESA Advanced Protein Crystallization Facility onboard the NASA Space Shuttle Orbiter during the IML-2 mission. CCD video monitoring was used to follow the crystallization process and evaluate the growth rate. During the mission some tetragonal crystals were observed moving over distances of up to 200 μm. This was correlated with microgravity disturbances caused by firings of vernier jets on the Orbiter. Growth-rate measurement of a stationary crystal (which had nucleated on the growth reactor wall) showed spurts and lulls correlated with an onboard activity: astronaut exercise. The stepped growth rates may be responsible for the residual mosaic block structure seen in crystal mosaicity and topography measurements.

AB - Lysozyme has been crystallized using the ESA Advanced Protein Crystallization Facility onboard the NASA Space Shuttle Orbiter during the IML-2 mission. CCD video monitoring was used to follow the crystallization process and evaluate the growth rate. During the mission some tetragonal crystals were observed moving over distances of up to 200 μm. This was correlated with microgravity disturbances caused by firings of vernier jets on the Orbiter. Growth-rate measurement of a stationary crystal (which had nucleated on the growth reactor wall) showed spurts and lulls correlated with an onboard activity: astronaut exercise. The stepped growth rates may be responsible for the residual mosaic block structure seen in crystal mosaicity and topography measurements.

UR - https://www.scopus.com/pages/publications/0030831647

U2 - 10.1107/S0907444997007968

DO - 10.1107/S0907444997007968

M3 - Article

C2 - 11540584

AN - SCOPUS:0030831647

SN - 0907-4449

VL - 53

SP - 747

EP - 755

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 6

ER -

CCD video observation of microgravity crystallization of lysozyme and correlation with accelerometer data

Abstract

Access to Document

Other files and links

Fingerprint

Cite this