Calcium transport in isolated bone cells II. Calcium transport studies

Rosemary Dziak; John S. Brand

doi:10.1002/jcp.1040840110

Calcium transport in isolated bone cells II. Calcium transport studies

Rosemary Dziak
, John S. Brand

Department of Oral Biology

University of Rochester

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Calcium transport was studied in bone cells isolated from fetal rat calvaria. ⁴⁵Ca uptake experiments revealed an active component of calcium exchange. Calcium uptake was inhibited by iodoacetamide, DNP, CCCP and oligomycin and appeared to be dependent on medium phosphate concentration. Initial influx values exhibited saturation kinetics from 0.6 mM to 1.5 mM extracellular calcium. Efflux of ⁴⁵Ca from loaded cells increased in the presence of iodoacetamide, DNP and CCCP. Incubation of the cells af 4° C inhibited both influx and efflux of calcium. Parathyroid hormone had no consistent effect on calcium uptake although characteristic increases in cyclic AMP levels were seen with the hormone. Calcitonin appeared to cause a transient increase in calcium uptake.

Original language	English
Pages (from-to)	85-96
Number of pages	12
Journal	Journal of Cellular Physiology
Volume	84
Issue number	1
DOIs	https://doi.org/10.1002/jcp.1040840110
State	Published - Aug 1974

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title = "Calcium transport in isolated bone cells II. Calcium transport studies",

abstract = "Calcium transport was studied in bone cells isolated from fetal rat calvaria. 45Ca uptake experiments revealed an active component of calcium exchange. Calcium uptake was inhibited by iodoacetamide, DNP, CCCP and oligomycin and appeared to be dependent on medium phosphate concentration. Initial influx values exhibited saturation kinetics from 0.6 mM to 1.5 mM extracellular calcium. Efflux of 45Ca from loaded cells increased in the presence of iodoacetamide, DNP and CCCP. Incubation of the cells af 4° C inhibited both influx and efflux of calcium. Parathyroid hormone had no consistent effect on calcium uptake although characteristic increases in cyclic AMP levels were seen with the hormone. Calcitonin appeared to cause a transient increase in calcium uptake.",

author = "Rosemary Dziak and Brand, \{John S.\}",

year = "1974",

month = aug,

doi = "10.1002/jcp.1040840110",

language = "English",

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pages = "85--96",

journal = "Journal of Cellular Physiology",

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AU - Dziak, Rosemary

AU - Brand, John S.

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AB - Calcium transport was studied in bone cells isolated from fetal rat calvaria. 45Ca uptake experiments revealed an active component of calcium exchange. Calcium uptake was inhibited by iodoacetamide, DNP, CCCP and oligomycin and appeared to be dependent on medium phosphate concentration. Initial influx values exhibited saturation kinetics from 0.6 mM to 1.5 mM extracellular calcium. Efflux of 45Ca from loaded cells increased in the presence of iodoacetamide, DNP and CCCP. Incubation of the cells af 4° C inhibited both influx and efflux of calcium. Parathyroid hormone had no consistent effect on calcium uptake although characteristic increases in cyclic AMP levels were seen with the hormone. Calcitonin appeared to cause a transient increase in calcium uptake.

UR - https://www.scopus.com/pages/publications/0016209541

U2 - 10.1002/jcp.1040840110

DO - 10.1002/jcp.1040840110

M3 - Article

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AN - SCOPUS:0016209541

SN - 0021-9541

VL - 84

SP - 85

EP - 96

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

IS - 1

ER -

Calcium transport in isolated bone cells II. Calcium transport studies

Abstract

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