Calcium transport in isolated bone cells I. Bone cell isolation procedures

Rosemary Dziak; John S. Brand

doi:10.1002/jcp.1040840109

Calcium transport in isolated bone cells I. Bone cell isolation procedures

Rosemary Dziak
, John S. Brand

Department of Oral Biology

University of Rochester

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

Bone cells were isolated by collagenase‐hyaluronidase digestion of 19–20 day‐old fetal rat calvaria. It is shown that contamination from calcified matrix can give erroneously high values for total cell calcium and can mask intracellular calcium exchange. Filtration of the cell suspension through 35 μ mesh nylon net and a five minute treatment with a cold pH 6.0 isotonic salt solution removed the contamination. Total calcium for cells prepared in this manner was 16.8 mmoles/kg wet weight. ⁴⁵Ca uptake studies revealed an active component of calcium exchange.

Original language	English
Pages (from-to)	75-83
Number of pages	9
Journal	Journal of Cellular Physiology
Volume	84
Issue number	1
DOIs	https://doi.org/10.1002/jcp.1040840109
State	Published - Aug 1974

Access to Document

10.1002/jcp.1040840109

Cite this

@article{4dd1cca5c8ac4da3b16e7976a18a5dd6,

title = "Calcium transport in isolated bone cells I. Bone cell isolation procedures",

abstract = "Bone cells were isolated by collagenase‐hyaluronidase digestion of 19{\textendash}20 day‐old fetal rat calvaria. It is shown that contamination from calcified matrix can give erroneously high values for total cell calcium and can mask intracellular calcium exchange. Filtration of the cell suspension through 35 μ mesh nylon net and a five minute treatment with a cold pH 6.0 isotonic salt solution removed the contamination. Total calcium for cells prepared in this manner was 16.8 mmoles/kg wet weight. 45Ca uptake studies revealed an active component of calcium exchange.",

author = "Rosemary Dziak and Brand, \{John S.\}",

year = "1974",

month = aug,

doi = "10.1002/jcp.1040840109",

language = "English",

volume = "84",

pages = "75--83",

journal = "Journal of Cellular Physiology",

issn = "0021-9541",

publisher = "Wiley-Liss Inc.",

number = "1",

}

TY - JOUR

T1 - Calcium transport in isolated bone cells I. Bone cell isolation procedures

AU - Dziak, Rosemary

AU - Brand, John S.

PY - 1974/8

Y1 - 1974/8

N2 - Bone cells were isolated by collagenase‐hyaluronidase digestion of 19–20 day‐old fetal rat calvaria. It is shown that contamination from calcified matrix can give erroneously high values for total cell calcium and can mask intracellular calcium exchange. Filtration of the cell suspension through 35 μ mesh nylon net and a five minute treatment with a cold pH 6.0 isotonic salt solution removed the contamination. Total calcium for cells prepared in this manner was 16.8 mmoles/kg wet weight. 45Ca uptake studies revealed an active component of calcium exchange.

AB - Bone cells were isolated by collagenase‐hyaluronidase digestion of 19–20 day‐old fetal rat calvaria. It is shown that contamination from calcified matrix can give erroneously high values for total cell calcium and can mask intracellular calcium exchange. Filtration of the cell suspension through 35 μ mesh nylon net and a five minute treatment with a cold pH 6.0 isotonic salt solution removed the contamination. Total calcium for cells prepared in this manner was 16.8 mmoles/kg wet weight. 45Ca uptake studies revealed an active component of calcium exchange.

UR - https://www.scopus.com/pages/publications/0016168560

U2 - 10.1002/jcp.1040840109

DO - 10.1002/jcp.1040840109

M3 - Article

C2 - 4367530

AN - SCOPUS:0016168560

SN - 0021-9541

VL - 84

SP - 75

EP - 83

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

IS - 1

ER -

Calcium transport in isolated bone cells I. Bone cell isolation procedures

Abstract

Access to Document

Other files and links

Fingerprint

Cite this