Bradford assay for determining protein concentration

Clara L. Kielkopf; William Bauer; Ina L. Urbatsch

doi:10.1101/pdb.prot102269

Bradford assay for determining protein concentration

Clara L. Kielkopf
, William Bauer
, Ina L. Urbatsch

Pediatrics

Research output: Contribution to journal › Article › peer-review

520 Scopus citations

Abstract

The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.

Original language	English
Pages (from-to)	136-138
Number of pages	3
Journal	Cold Spring Harbor Protocols
Volume	2020
Issue number	4
DOIs	https://doi.org/10.1101/pdb.prot102269
State	Published - Apr 2020

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title = "Bradford assay for determining protein concentration",

abstract = "The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.",

author = "Kielkopf, \{Clara L.\} and William Bauer and Urbatsch, \{Ina L.\}",

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AB - The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.

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JO - Cold Spring Harbor Protocols

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Bradford assay for determining protein concentration

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