@inbook{5c10ab6d5c9c4f019d3040b695f9b00c,
title = "Bioorthogonal Labeling and Click-Chemistry-Based Visualization of the Tannerella forsythia Cell Wall",
abstract = "The objective of this chapter is to provide a detailed protocol for the peptidoglycan (cell wall) labeling of the periodontal pathogen Tannerella forsythia and the development of a laboratory-safe Escherichia coli strain utilizing the N-acetylmuramic acid recycling enzymes AmgK, N-acetylmuramate/N-acetylglucosamine kinase, and MurU, N-acetylmuramate alpha-1-phosphate uridylyltransferase, from T. forsythia. The procedure involves bioorthogonal labeling of bacterial cells with an azido-modified analog of the amino sugar, N-acetylmuramic acid, through “click chemistry” with a fluorescent dye. The protocol is suitable for the generation of fluorescently labeled peptidoglycan molecules for applications in the study of bacterial and peptidoglycan trafficking in the host cells and cell wall recycling in complex microbiomes.",
keywords = "Bioorthogonal, Cell wall recycling, Click chemistry, Mass spectrometry, Microscopy, Peptidoglycan, Tannerella forsythia",
author = "Hyland, \{Stephen N.\} and Sreedevi Chinthamani and Sushanta Ratna and Wodzanowski, \{Kimberly A.\} and Sandles, \{Liam Michael D.\} and Kiyonobu Honma and Catherine Leimkuhler-Grimes and Ashu Sharma",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3491-2\_1",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "1--16",
booktitle = "Methods in Molecular Biology",
}